Biotoxins are widely distributed in animals, plants, and microorganisms, functioning as “chemical weapons” of proteins, peptides, or small molecules that evolved for predation, defense, and competition. Compared with general chemical toxins, biotoxins exhibit high potency, strong specificity, and remarkable molecular diversity. While posing potential threats to human health, they also provide unique value in elucidating disease mechanisms and inspiring drug development. Representative drugs such as captopril, botulinum toxin, ziconotide, and GLP-1 receptor agonists mark milestones in the therapeutic application of toxins. To date, over one hundred biotoxin-derived drug candidates have entered clinical trials across multiple major diseases. However, this field still faces challenges, including low efficiency in resource discovery, limited structural and mechanistic insights, inherent toxicity, and constraints in synthesis and modification technologies. Looking forward, advances in multi-omics, artificial intelligence, and synthetic biology will drive efficient toxin discovery, detoxification strategies, and precision applications, ultimately promoting a closed-loop progression from basic research to clinical translation.

Lung cancer poses a serious threat to global public health security. Chemotherapy, as the main strategy for cancer treatment, faces challenges such as high toxicity and drug resistance. Anticancer peptides have the potential of being developed into new anticancer drugs due to their advantages of broad-spectrum anticancer activity, rapid action, and difficulty in generating drug resistance, but they also face shortcomings such as weak activity and strong toxic side effects. The weakly acidic microenvironment of tumors (pH 6.5-6.8) provides a good idea for the design of anticancer peptides of high-efficiency and low-toxicity. Previously, we designed the acid-sensitive antibacterial peptide pHly-1 using the wolf spider (Lycosa singoriensis) toxin Lycosin-I as a template. In this study, we found that pHly-1 also had acid-sensitive anticancer activity. Further alanine scanning analysis of pHly-1 was carried out, and we obtained a mutant pHTP-2 with better acid sensitivity, whose IC₅₀ (half maximal inhibitory concentration) against A549 cells was 15.68 μmol/L at pH 6.6 and was greater than 100 μmol/L at pH 7.4. At pH 6.6, pHTP-2 could act on various lung cancer cell lines and induce the death of A549 cells by rapid lysis; at pH 7.4, 500 μmol/L pHTP-2 had weak toxicity to red blood cells (the hemolysis rate was approximately 38%) and primary myocardial cells (the inhibition rate was 49.7%, with P< 0.05). Analysis of its charge, particle size, morphology, and secondary structure showed that at pH 6.6, the histidine in the sequence of pHTP-2 was protonated, increasing the positive charge (P<0.01), decreasing the hydrated particle size (P<0.05) and forming an α-helical structure to induce membrane lysis of A549 cells. At pH 7.4, it was deprotonated, the positive charge decreases, a β-sheet structure was formed and self-aggregation occurred, limiting its effect on the A549 cell membrane and showing weak activity. In summary, pHTP-2 could respond to the weakly acidic microenvironment of tumors to exert selective cytotoxic activity, effectively overcoming the shortcomings of anticancer peptides such as low efficiency and high toxicity. Our findings suggest that it is a high-quality lead molecule for anticancer drugs.

Kv1.3, a voltage-gated potassium channel, is highly expressed in T lymphocytes, the nervous system, and vascular smooth muscle cells. It plays a critical role in membrane excitability and electrical signal transduction, serving as an important target for studying T-cell function and providing a promising direction for developing therapeutics against autoimmune and inflammatory diseases. Therefore, the development of specific inhibitors of Kv1.3 channel has emerged as a novel therapeutic strategy for these disorders. In this study, we isolated and purified a novel Kv1.3-inhibitory peptide toxin, LmKTx13, from the venom of the scorpion Lychas mucronatus using reversed-phase high-performance liquid chromatography (RP-HPLC). LmKTx13 consists of 38 amino acid residues, including six cysteines that form three disulfide bonds. Whole-cell patch-clamp recordings revealed that LmKTx13 potently inhibited Kv1.3 with anIC₅₀ of 7.92 ± 3.0 nmol/L. Selectivity analysis showed that 2 μmol/L LmKTx13 also inhibited Kv1.2 and Kv1.7, but exhibited no significant effects on other potassium channel subtypes or voltage-gated sodium channels. Further investigation into the mechanism demonstrated that LmKTx13 acts as a pore-blocking inhibitor of Kv1.3. By analyzing the effects of LmKTx13 on Kv1.3 channel gating kinetics and performing sequence alignment of the pore regions of Kv1.3 and Kv1.5, we constructed site-directed mutants and identified the pore region of Kv1.3 as the critical binding site for LmKTx13. Key residues involved in the interaction included T425, G427, and H451. In summary, we discovered a novel pore-blocking Kv1.3 inhibitor, LmKTx13, from L. mucronatus venom, which exhibits high affinity and selectivity for Kv1.3. These findings highlight its potential as a potential lead molecule for developing Kv1.3-targeted therapeutics.

Wound repair and infection have long posed significant challenges in clinical medicine. Amphibians, adapting to complex environments through long-term natural selection, have evolved skin systems with remarkable abilities to resist external damage and promote rapid wound healing, making them an important source for discovering candidate molecules for wound healing. In this study, a novel peptide composed of 22 amino acids, with the sequence "VGKAGLETAACKATNSCFNIDW" and a molecular weight of 2299.6 D, was screened from the cDNA library of Odorrana tormota and named PN-VW22. The peptide was synthesized by solid-phase synthesis, and its effects on cell proliferation were evaluated using the CCK-8 assay and scratch wound healing assay in human keratinocytes (HaCaT) and mouse embryonic fibroblasts (NIH3T3). Antibacterial activity was assessed by determining the minimum inhibitory concentration (MIC). Results showed that PN-VW22 at various concentrations had no significant effect on the cell viability in NIH3T3 cells (P>0.05), but significantly enhanced HaCaT cell viability at 0.5 μmol/L (P＜0.001). Meanwhile, PN-VW22 induced cell proliferation and promoted wound healing in HaCaT cells, with a healing rate of 64.44% after 24h incubation at 0.5 μmol/L. Furthermore, PN-VW22 exhibited antibacterial activity against Staphylococcus aureus with an MIC value of 13.92 μmol/L. In sum, this study identified PN-VW22 as a novel bifunctional peptide with both wound repair and anti-infective properties, providing a new toxin peptide template for the development of wound healing drugs.

Pancreatic cancer has emerged as one of the most challenging malignancies worldwide, with its high resistance to chemotherapy being the primary cause of treatment failure. Therefore, enhancing the chemosensitivity of pancreatic cancer has become a major focus of current research. In this study, we investigated how Bufotaline, a bufadienolide extracted from the traditional Chinese medicine toad venom, exhibits its antitumor activity. Specifically, we explored the potential of Bufotaline to enhance the chemosensitivity of pancreatic cancer cells to Adriamycin and elucidated its underlying molecular mechanisms. Using CCK-8 and colony formation assays, we demonstrated that Bufotaline enhances the inhibitory effect of Adriamycin on the survival of pancreatic cancer cell lines Patu-8988T, Aspc-1, and Patu-8988S. Notably, Bufotaline treatment reduced the IC₅₀ of Adriamycin in drug-resistant pancreatic cancer cells to levels comparable to those in non-resistant cells. Results from Western blot, immunofluorescence, comet assay, and TUNEL assays revealed that Bufotaline promotesAdriamycin-induced DNA damage in pancreatic cancer cells. RNA-seq analysis of Patu-8988T cells treated with Adriamycin alone or in combination with Bufotaline showed significant changes in gene expression, and qRT-PCR analysis further confirmed that Bufotaline downregulates the expression of DNA damage repair proteins NBS1 and RAD50. Moreover, Western blot analysis revealed that Bufotaline reduces the levels of DNA damage response repair proteins, and Immunofluorescence experiments indicated that Bufotaline inhibits the activation of the ATM/CHK2 signaling pathway. Finally, in a subcutaneous xenograft mouse model, the combination of Adriamycin and Bufotaline treatment significantly suppressed pancreatic cancer cell growth. In conclusion, Bufotaline enhances Adriamycin-induced chemosensitivity in pancreatic cancer cells; the combination of Adriamycin and Bufotaline downregulates the expression of DNA damage response repair proteins NBS1 and RAD50, and inhibits the ATM/CHK2-mediated DDR signaling pathway, thereby delaying DNA damage repair.

CRM197 (cross-reacting material 197), a naturally occurring mutant of diphtheria toxin, is a safe and effective vaccine vector and extensively used on developing conjugate or combined vaccines. The mutant loses its enzymatic activity, but fully retains its receptor-binding ability and immunogenicity. In current work, the diphtheria toxin mutant CRM197 and its fusion proteins with the receptor-binding domain of botulinum neurotoxin serotype E (EHc) were developed using genetic engineering technology. These recombinant proteins were confirmed by Western blotting and SDS-PAGE. BALB/c mice were immunized with the CRM197-EHc and EHc-CRM197 fusion proteins, and their immunogenicity was evaluated. These two fusion protein molecules, CRM197-EHc and EHc-CRM197, as subunit vaccines, elicited a robust humoral immune response targeting both CRM197 and EHc antigens in the immunized mice. Compared to the mixture of CRM197 and EHc, the mice vaccinated with the fusion proteins (CRM197-EHc and EHc-CRM197) induced higher levels of anti-CRM197 antibodies, and the mice vaccinated with EHc-CRM197 also generated strongest anti-EHc antibodies. Consequently, as a carrier molecule in the fusion protein vaccine, EHc enhances the immunogenicity of CRM197 molecules. Likewise, CRM197 boosts the immunogenicity of EHc in the EHc-CRM197 fusion protein.

The bacterial ADP-ribosylating exotoxins are produced by bacteria and infect different human body tissues. Herein, we investigated the molecular evolution of AB₅-type bacterial toxins expressed by Vibrio cholerae and other bacteria with similar invasion mechanisms to interpret the co-evolutionary history of V. cholerae cholera toxin (CT) and their hosts, aiming to reveal the causes of its transdermal immunogenicity. We elaborated on the intracellular toxicity mechanisms of CT, including ganglioside receptor-mediated endocytosis and hyperactivation of the cAMP pathway, as well as the behavioral traces of related bacteriophages within the genomes of these bacteria. Models such as the relatively decoupled evolution of A and B subunits of CT and the evolutionary coupling of transdermal and mucosal immunity were summarized. Furthermore, we described mechanisms including phage-mediated horizontal gene transfer (exemplified by Vibrio phage CTXΦ), toxin targeting variation, expansion of molecular recognition domains, and functional adaptive evolution of the toxins. In this research, we employed bioinformatic tools to construct phylogenetic trees and analyze genetic variations in the amino acid sequences of toxin A/B subunits and proteins of secretion systems. Tajima’s test was utilized to quantify genetic distance, diversity, and neutral selection pressure. Key findings include: (1) a “decoupled evolution” mode for the A and B subunits of CT, with the B subunit under stronger negative selection; (2) horizontal gene transfer mediated by CTXΦ and other phages drives the cross-species spread of toxin genes; (3) the interaction between the toxin co-regulated pilus (TCP) and the TLR-5 (Toll-like receptor 5) promotes the transdermal immunogenicity of the CT B subunit. These findings suggest the role of “toxin-host arm race” co-evolution, and are consistent with the hypothesis of intergenerational transmission of immune memory in CT evolution, thereby providing theoretical support for further research into the biological mechanisms and co-evolutionary history of AB₅-type bacterial toxins.

The application of botulinum neurotoxin (BoNT) in cosmetic procedures of the head and neck is well established. In recent years, however, its therapeutic scope has significantly expanded to include functional and pathological disorders within this anatomical region. This article reviews the use of BoNT as an important treatment modality for various head and neck disorders, encompassing movement disorders (such as laryngeal, craniocervical, middle ear, oromandibular, and cervical dystonia), peripheral nerve dysfunction (including multiple system atrophy, migraine, synkinesis and spasm following facial nerve palsy), upper aerodigestive tract dysfunction(e.g., post-laryngectomy complications, cricopharyngeal dysphagia, pharyngeal pouch, and retrograde cricopharyngeal dysfunction), and autonomic nervous disorders (such as sialorrhea and gustatory sweating syndrome). By leveraging its highly specific neuromodulatory effects, BoNT provides a reversible, minimally invasive, and potent therapeutic alternative for conditions often refractory to conventional pharmacological or surgical interventions. This review systematically examines the mechanisms of action, injection techniques, clinical efficacy, and safety profiles of BoNT in the above-mentioned diseases, and discusses future applications, aiming to offer comprehensive evidence-based guidance for clinical practice to otolaryngologists-head and neck surgeons.

Toxin-antitoxin (TA) systems serve as central hubs of bacterial adaptive regulation and play critical roles in the pathogenesis of Mycobacterium tuberculosis (M.tb) and Bordetella pertussis (B. pertussis). This review summarizes the functional evolution and therapeutic potential of TA systems in M. tb and B. pertussis. It systematically outlines the molecular mechanisms and pathogenic functions of TA systems in these two pathogens. M.tb relies on typeⅡ TA systems (e.g., VapBC, MazEF) to drive persister formation and antibiotic tolerance through toxin-mediated ribonuclease activity that cleaves host nucleic acids or DarT/DarG-mediated DNA modification. In contrast, B. pertussis utilizes a unique temperature-sensing PhtA/PhtB system to release adenylate cyclase toxin, which targets the host cAMP signaling pathway to achieve immune evasion. Both pathogens employ TA toxins to suppress host defenses—such as VapC cleaving tRNA and RelE degrading NF-κB components. Their high-frequency mutation sites (e.g., the VapC47-Ser46Leu mutation frequency >50 000 in M. tuberculosis) reveal strong positive selection pressure, closely associated with persister phenotypes and virulence evolution. This review further discusses therapeutic strategies, including small-molecule inhibitors targeting toxin-antitoxin interactions, TA-deletion attenuated vaccines, and antitoxin-based immunization approaches. Finally, it highlights the need for future research to elucidate TA-host interaction networks and develop nanocarrier delivery technologies to advance breakthroughs in precision therapy for tuberculosis and pertussis.

Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized primarily by pancreatic β-cell dysfunction and insulin resistance. Beyond impaired glucose homeostasis, the glucotoxicity resulting from dysregulated glucose metabolism can lead to complications such as diabetic nephropathy, diabetic coronary heart disease, and diabetic cognitive dysfunction, posing severe threats to patients′ health. Therefore, enhancing glucose metabolism and improving insulin resistance are critical strategies for T2DM prevention and treatment. Currently, the primary therapeutic approaches for T2DM include oral hypoglycemic agents and exogenous insulin injections. However, these methods face challenges such as unclear diagnostic markers, suboptimal therapeutic efficacy, and drug-related side effects. Consequently, the development of more precise and effective treatment strategies remains a top priority in clinical research. MicroRNA (miRNA) is a short non-coding RNA that regulates gene transcription. It has been reported to exhibit significant alterations even before the onset of overt pathological features like hyperglycemia, suggesting their potential as early diagnostic biomarkers for T2DM to address current issues like delayed diagnosis and disease progression due to missed optimal treatment windows. Moreover, miRNAs can modulate the expression of pathways involved in glucose metabolism, thereby improving insulin resistance and maintaining glucose homeostasis while avoiding side effects associated with conventional therapies, such as reduced bone density and localized fat accumulation. This positions miRNAs as a promising avenue for precise and efficient T2DM management. This article systematically elucidates the molecular mechanisms by which miRNAs regulate glucose metabolism-related pathways, providing a theoretical foundation and reference for future clinical research.

O-glycosylation (including mucin-type O-glycosylation and O-GlcNAcylation), as a critical post-translational modification (PTM), regulates protein function, stability, and subcellular localization through the addition of glycan chains to serine or threonine residues, which participates in cellular signaling, metabolic regulation, and stress responses. DNA damage refers to the disruption of genomic integrity caused by endogenous factors (e.g., metabolic byproducts, replication errors) or exogenous agents (e.g., radiation, chemical substances), leading to carcinogenesis, aging, and genetic disorders. To counteract DNA lesions, organisms have evolved the DNA damage response (DDR) system, which orchestrates complex protein networks to detect DNA damage and facilitate repair processes. Emerging evidence indicates that O-glycosylation can modulate DDR by influencing the activity, localization, and interactions of DNA repair-associated proteins. However, the precise mechanisms underlying O-glycosylation-mediated DDR remain to be clarified. This review systematically summarizes: (1) the biosynthetic pathways of mucin-type O-glycosylation and O-GlcNAcylation, the cascade reactions in DDR; and (2) current research advances regarding O-glycosylation in tumor-associated DDR. Furthermore, we propose novel mechanistic perspectives and therapeutic strategies targeting O-glycosylation-mediated DDR dysregulation in malignancies, aiming to provide a theoretical basis for tumor treatment.

Wound healing is a dynamic physiological process involving haemostasis, inflammation, proliferation and tissue remodeling. Skin injuries, such as diabetic foot ulcers, venous ulcers, and pressure ulcers, are difficult to heal and impose a serious physical and psychological burden on patients, and traditional treatments are difficult to address such problems. In recent years, Chinese herbal medicine-derived extracellular vesicles (CHMEVs) have shown promising potential in the field of wound repair and drug delivery due to their excellent biocompatibility, low immunogenicity and high safety. CHMEVs are nano-like particles enriched with herbal small molecule compounds, proteins and metabolites, and are able to cross biological barriers and effectively regulate intercellular communication to promote tissue repair. In this review, the isolation and extraction methods of CHMEVs and their roles in wound repair are reviewed, and the prospects and challenges of their clinical applications are discussed. The combination of engineered modification and biomaterials of CHMEVs further expands their potential for application in precision medicine and provides a new idea for the treatment of hard-to-heal wounds in the future.

Lactylation modification is a new type of protein post-translational modification, which mediates the covalent binding of lactic acid groups to lysine residues through amide bonds, thus changing protein function and intracellular signal transduction process. Lactylation modifications can be broadly categorized into two types: histone lactylation and non-histone lactylation, both of which are dynamically and precisely regulated by the "Writer-Eraser" enzyme system. Among them, non-histone lactylation, mainly regulated by enzymes such as AARS1 and SIRT3, plays an important role in the progression of many diseases, including tumor metabolic reprogramming, ROS stress and signal pathway regulation. Especially in tumors, non-histone lactylation is closely related to tumor proliferation, immune escape and drug resistance. Therefore, an in-depth study of the role of non-histone lactylation in the progression of tumors is expected to provide new targets and strategies for the accurate diagnosis and treatment of tumors. It is noteworthy that in the context of non-histone lactylation modification, the interference effect of acetylation modification cannot be ignored. Lactylation and acetylation share similar "writer" and "eraser" enzymes and exhibit overlapping modification sites, suggesting the possibility of functional crosstalk between the two. Due to the current lack of specific editing tools targeting lysine lactylation, it remains challenging to definitively determine whether lactylation plays a predominant regulatory role. This article reviews the research progress of non-histone lactylation in tumors in recent years.

Hepatocellular carcinoma (HCC), which is essentially primary liver cancer, is closely related to CD8^＋ T cell immune infiltration and immune suppression. We constructed a CD8⁺ T cells related risk score model to predict the prognosis of HCC patients and provided therapeutic guidance based on the risk score. Using integrated bulk RNA sequencing (RNA-seq) and single-cell RNA sequencing (scRNA-seq) datasets, we identified stable CD8⁺ T cell signatures. Based on these signatures, a 3-gene risk score model, comprised of KLRB1, RGS2, and TNFRSF1B was constructed. The risk score model was well validated through an independent external validation cohort. We divided patients into high-risk and low-risk groups according to the risk score and compared the differences in immune microenvironment between these two groups. Compared with low-risk patients, high-risk patients have higher M2-type macrophage content (P<0.0001) and lower CD8⁺ T cells infiltration (P<0.0001). High-risk patients predict worse response to immunotherapy treatment than low-risk patients (P<0.01). Drug sensitivity analysis shows that PI3K-β inhibitor AZD6482 and TGFβRII inhibitor SB505124 may be suitable therapies for high-risk patients, while the IGF-1R inhibitor BMS-754807 or the novel pyrimidine-based anti-tumor metabolic drug Gemcitabine could be potential therapeutic choices for low-risk patients. Moreover, expression of these 3-gene model was verified by immunohistochemistry. In summary, the establishment and validation of a CD8⁺ T cell-derived risk model can more accurately predict the prognosis of HCC patients and guide the construction of personalized treatment plans.

Inflammatory response, immunosuppression, and drug sensitivity have been reported to have a significant correlation with the disulfidptosis levels in cancer patients. However, the value of disulfidptosis in colorectal cancer therapy remains unclear. Therefore, we classified the CRC cells into different cell types using single-cell sequencing data and cell-specific markers and analyzed their relationship with the cell disulfidptosis level. We found that the high disulfidptosis regions were concentrated in epithelial-like CRC cells. Further exploration using the disulfidptosis and programmed cell death 1 inhibitor therapy treated differential expression genes indicated that CRC patients with high disulfidptosis levels exhibited a lower risk profile and increased sensitivity to immunotherapy. By using the spatial transcriptomic analysis, we found that ubiquinol-cytochrome c reductase core protein 1 (UQCRC1), a disulfidptosis-related gene, is highly expressed in epithelial-like CRC cells and co-localized with immune-infiltrated tumor regions. Additional bioinformatic analyses and experimental validation further confirmed that UQCRC1 was downregulated in CRC tissues. Overexpression of UQCRC1 suppressed CRC cell proliferation and migration.These findings indicate that UQCRC1 is a potential target for CRC diagnosis and treatment.

Human non-histone lysine crotonylation plays crucial roles in biological activities. However, traditional wet-lab experiments are time-consuming and labor-intensive, making computational prediction methods increasingly popular in recent years. Despite the biological importance of lysine crotonylation, there are relatively few studies on human non-histone proteins. In this study, we developed an ensemble deep learning predictor named iHNHC-RsFPN by constructing a Residual Pyramid Network (RsFPN). First, three feature extraction methods were employed to encode sequence samples. Next, weak classifiers based on RsFPN were individually trained for different feature types. Finally, these weak classifiers were integrated to build a robust final predictor. Independent test results demonstrated that iHNHC-RsFPN achieved outstanding performance across four key metrics: sensitivity (Sn = 0.8580), specificity (Sp = 0.7463), accuracy (Acc = 0.7798), and Matthews correlation coefficient (MCC = 0.5586). Comparative experiments revealed that iHNHC-RsFPN significantly improved prediction accuracy for human non-histone crotonylation sites over existing methods. Additionally, we established a user-friendly web server (http://www.lzzzlab.top/ihnc/) that provides straightforward prediction services without complex calculations, facilitating further research for experts in related fields.

In-fusion cloning technology, as a revolutionary and efficient molecular biology tool, has been applied in multiple research fields such as basic biology, biotechnology, and biomedicine. In this article, we introduce a teaching reform project suitable for undergraduate students in the course of “Molecular Biology Laboratory”, which utilizes in-Fusion cloning technology to construct a prokaryotic expression vector for alkaline phosphatase mutant genes. Through specific teaching cases, we systematically explored the design and implementation of experimental projects, and focused on analyzing the key and difficult points of the teaching content. Our teaching practice has found that the implementation of this educational reform project has achieved very good results in enhancing students’ core biological literacy, bioinformatics skills, research thinking, and innovation abilities. At the same time, the application of this technology can significantly improve the quality of experimental teaching, providing new ideas and practical references for promoting the reform and innovation of National First-Class Courses.