1)Department of Biochemistry and Molecular Biology: 2) Dep artment of Human Anatomy, Histology and Embryology: 3)Department of Physiology and Pathophysiology:Peking University Health Science Center, Beijing 100083, China
Abstract：Hepatic steatosis, featured in hepatic accumulation in lipid, has been recognized as a promising stage toward to fatty liver, initiated by such as chronic alcoholism, obesity or certain drug intoxication and etc., and finally leads to hepatic necrosis and cirrhosis. However, the underlying mechanism on the hepatic steatosis is poorly understood. Hepatic steatosis rats were induced by subcutaneous injections of carbon tetrachloride (CCl4) solution. The product increase of hepatic malonaldehyde was 68%, and higher level of GRP78 expression was found by RT-PCR and Western blot in the liver of rats treated by CCl4 Further s tudy using cultured HepG2 cells showed that CCl4 could lead to endoplasmic reticulum stress (ERS), which induced the expression and activation of sterol regulatory element binding protein-1 (SREBP-1).The results suggest that oxidative damage and endoplasmic reticu lum stress are implicated in the CCl4 induced hepatic steatosis. This ERS disbur dened by CCl4 in turn leads to translocation and binding of SREBP-1 to those promoter regions of lipogenic genes, which is assumed to enhance those lipogenic gene transcriptions responsible for triglyceride/ cholesterol biosynthesis and accumulation.
林胜利；卓德祥；王晓红；吴逸园；王珂；吴耀南；张卫光；李载权；唐朝枢. 氧化损伤与内质网应激在四氯化碳致大鼠肝脂肪变性中的作用机制[J]. 中国生物化学与分子生物学报, 2006, 22(05): 422-430.
LINSheng-Li；ZHUODe-Xiang；WANGXiao-Hong；WUYi-Yuan；WANGKe；WUYao-Nan:ZHANGWei-Guang ；LIZai-Ｑuan ；TANGChao-Shu. Oxidative Damage and ER Stress are Implicated in CCl4-induced SD Rat Hepatic Steatosis. Chinese Journal of Biochemistry and Molecular Biol, 2006, 22(05): 422-430.
