LIN Ya-Qiu, LI Rui-Wen, ZHENG Yu-Cai, JI Hong, LIU Mao-Qi
Chinese Journal of Biochemistry and Molecular Biol. 2011, 27(6): 554-560.
The cDNA sequence of heart fatty acid binding protein gene (H-FABP) was cloned from Schizothorax prenanti and Cyprinus carpio. The expression profile of H-FABP mRNA was analyzed using semi-quantitative RT-PCR. The intramuscular fat (IMF) content was measured to compare the function of H-FABP gene in fat deposition in S.prenanti and C.carpio which has different habitat. The results showed that the obtained cDNA fragments had a 402 bp ORF encoding 133 amino acids totally identical between Schizothorax prenanti and C.carpio, and the homology of amino acid sequences were 71.3% to 90.0% with Danio rerio, Salmo salar, Oncorhynchus mykiss, Fundulus heteroclitus and Anguilla japonica, respectively. The H-FABP gene expression profiles were observed in heart, muscle, fat, liver, brain, spleen, kidney tissues and gill in both species. The expression of H-FABP gene in live was significantly higher than that in other tissues studied (P<0.05), and high level was also exhibited in heart. The expression profile of H-FABP gene in muscle differed between S.prenanti and C.carpio, its expression level increased along with the growth in S.prenanti, showing higher mRNA level in fish with larger weight(500 g) than in smaller weights (50～60 g and 120～130 g, P<0.05), and its expression profile showed significant positive correlation with IMF content (R=0.370, P<0.05). However, the expression level of H-FABP gene in muscle decreased with the growth in C.carpio, showing higher mRNA level in fish with smaller weight(50～60 g) than in two larger weights (P<0.05), and exhibited significant negative correlation with IMF content (R=-7.083，P<0.01). The results suggest that the difference in linkage between the expression of HFABP gene and IMF content in muscles of S.prenanti and C.carpio might relate to their different habitat.
