Abstract：With the advent of the post-genomics era, gene chip and high-throughput sequencing have become two most important technologies in biochemistry and molecular biology. Compared with the traditional genetic methods, these two technologies have made new breakthroughs in the detection efficiency, accuracy and the degree of automation. The gene chip technology is a high-throughput, high-efficiency, and high-automation method. Its core technical protocols have already been widely popularized and used in industrial applications. Although the high-throughput DNA sequencing technology was established after gene chip, its development speed is extremely fast, especially in terms of technical updating and replacement. Continuous improvement makes its application in life sciences an insurmountable advantage. There are marked differences between these two methods by principle. But the application of the two technologies often blend together. This paper briefly discusses and compares the principles of the gene chip and high-throughput sequencing technologies and their utilizations in gene copy number variation, the intestinal microbiology and agriculture.
徐晓丽,林娟,鄢仁祥. 基因芯片与高通量测序技术的原理与应用的比较[J]. 中国生物化学与分子生物学报, 2018, 34(11): 1166-1174.
XU Xiao-Li, LIN Juan, YAN Ren-Xiang. Comparison of Principle and Application of the Gene Chip and High-throughput Sequencing Technologies. Chinese Journal of Biochemistry and Molecular Biol, 2018, 34(11): 1166-1174.
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