Ubiquitin-conjugating enzyme E2C (UBE2C) is closely related to the proliferation of various tumor cells, but its relationship with the occurrence and development of lung cancer is not clear. The roles of UBE2C in cellular proliferation, cell senescence and migration of A549 lung cancer cells were elucidated by RT-PCR, Western blotting, immunofluorescence, SA-β-Gal cell senescence staining, cell scratch and Trans-well assays. Transient over-expression or targeted silencing of UBE2C by gene modification technique was used. The results showed that the expression of UBE2C in lung cancer cells was significantly higher than that in normal cells. The levels of UBE2C mRNA and protein were significantly increased by 3.5-fold or decreased by 0.5-fold in A549 cells after transient over-expression or silence of UBE2C, leading to significant promotion or inhibition of cell proliferation, thereby reducing or increasing the rate of apoptosis. In addition, over-expression of UBE2C significantly inhibited cell senescence, whereas silence of UBE2C promoted cell senescence. Moreover, UBE2C significantly affected the mRNA and protein expression of metastasis related genes E-cadherin and vimentin, and further modulated cell migration. This study provided information for the roles of UBE2C in the development and progression of lung cancer.
基金资助:烟台市科技发展计划 (No 2015ZH082)和山东省自然科学基金资助项目 (No ZR2018QH004, ZR2016HB55, ZR2017PH067)
通讯作者:
郭纪伟
E-mail: guojiwei0510@163.com
引用本文:
郭纪伟,武艳,金丹,杜静,宫凯凯,苗双,杨丽娟. 泛素偶联酶2C促进肺癌细胞增殖和迁移与抑制细胞衰老[J]. 中国生物化学与分子生物学报, 2018, 34(6): 649-658.
GUO Ji-Wei1) *, WU Yan,JIN Dan, DU Jing, GONG Kai-Kai,MIAO Shuang, YANG Li-Juan. UBE2C Promotes Proliferation and Migration of Lung Cancer Cells and Inhibits Cell Senescence. Chinese Journal of Biochemistry and Molecular Biol, 2018, 34(6): 649-658.
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