Cloning and Characterization of a New Human CNA1 Transcript Variant
LIU Shi-Rao1), XIE Bo-Hong2), SUN Rui3), XIE Yun-Fei1)*
(1)College of Life Science and Technology, Henan Cultivating Key Laboratory of Hereditary Diseases and Molecular Targeted Drugs, Xinxiang Medical University, Xinxiang 453003,Henan,China;2)School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003 Henan,China;3)Department of Public Education, Zhengzhou Railway Vocational and Technical College, Zhengzhou 450052,China)
Calcineurin (CN) is the only Ca2+calmodulin (CaM) dependent protein serine/ threonine phosphatase consisting of a catalytic subunit, CNA, and a regulatory subunit, CNB. There are three subtypes of CNA, but CNAα is the most frequent one which is encoded by CNA1 gene. In the present study, a new variant of human CNA1 transcript, CNAα4, was isolated from a human fetal brain cDNA library. The CNAα4 lacks exon 2 of coding region and encodes a truncated protein of 454 amino acid residues. As a result, 67 amino acids are missed in the phosphatase catalytic domain. By in vitro binding assays, CNAα4 was found to have a comparable affinity to CaM as the largest isoform of CNAα, CNAα1. However, CNAα4 is more effective in activating nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) than CNAα1, suggesting that the fragment of Ala20-Thr86 may be dispensable for the catalytic function of CNAα. On the contrary, this deletion in CNAα4 may favor the binding of CN to NFAT and then promote the activity of CN.
刘世饶 解博红 孙瑞 谢云飞. 一种新的人CNA1基因转录本的克隆和功能鉴定[J]. 中国生物化学与分子生物学报, 2015, 31(9): 967-974.
LIU Shi-Rao, XIE Bo-Hong, SUN Rui, XIE Yun-Fei. Cloning and Characterization of a New Human CNA1 Transcript Variant. Chinese Journal of Biochemistry and Molecular Biol, 2015, 31(9): 967-974.
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