The Role of Small Non-coding RNA BSR0602 in the Regulation of Stress Response in Brucella melitensis
YUAN Jiu-Yun1), 3), GONG Chun-Li3), ZHUANG Yu-Bing3),LEI Shuang-Shuang3), CHEN Ze-Liang3), HUANG Ke-He1)*, WANG Yu-Fei2), 3)*
(1）College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;2）Department of laboratory medicine, The general hospital of Chinese people’s armed police forces, Beijing 100039, China;3）Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China)
BSR0602 is a small non-coding RNA located in chromosome II of Brucella melitensis, which is proven to be associated with Brucella intracellular survival. To further study the role of BSR0602 in the regulation of stress response and determine genes regulated by this sRNA, the proteomes of wildtype and BSR0602 overexpression mutant were compared by using twodimensional polyacrylamide gel electrophoresis (2DPAGE). The differentially expressed proteins were identified by MALDI-TOF-MS. Most of these proteins participate in amino acid, inorganic ion, and carbohydrate transport and metabolism. Of note is that stress response protein HtrA (BMEI0613) and SodA (BMEI1367) were downregulated when BSR0602 was overexpressed. Results of qRT-PCR and Western blot confirmed that BSR0602 inhibition the transcription of sodA. Further phenotype assay demonstrated that BSR0602 overexpression strain were sensitive to endogenous superoxide and H2O2. As a posttranscriptional regulator, small non-coding RNA BSR0602 may play a role in Brucella intracellular survival and environment stress adaption by modulating expression of stress response proteins.
袁久云 龚春丽 庄妤冰 雷霜霜 陈泽良 黄克和 王玉飞. 非编码小RNA BSR0602在布鲁菌环境适应能力中的调控作用[J]. 中国生物化学与分子生物学报, 2015, 31(9): 989-998.
YUAN Jiu-Yun, GONG Chun-Li, ZHUANG Yu-Bing,LEI Shuang-Shuang, CHEN Ze-Liang, HUANG Ke-He, WANG Yu-Fei. The Role of Small Non-coding RNA BSR0602 in the Regulation of Stress Response in Brucella melitensis. Chinese Journal of Biochemistry and Molecular Biol, 2015, 31(9): 989-998.
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