Abstract：Inflammation induces the generation reactive oxygen species (ROS) and tissue oxidative stress during the cause of chronic inflammatory disease and cancer in the digestive tract. The nuclear factorkappaB (NF-κB) signal pathway plays important roles in intestinal oxidative stress, colorectal inflammation and cancers. In this study, a small interference RNA (siRNA) of 60 base pairs targeting the NF-κB p65 gene was chemically synthesized, as well as cloned into a pSUPER plasmid. The plasmid was transfected into HCT116 cells. Western blot and MTT assays were conducted to determine the expression of NF-κB p65 and cell viability. The results demonstrated that downregulation of p65 increased the production of ROS and sensitized the oxidative damage induced by H2O2. It suggested that inhibition of NF-κB signal pathway might sensitize cancer cells to oxidative stresses.
周雪宏 卢瑶 曹继祥 李淑艳. NF-κB p65的敲减加重结肠上皮HCT116细胞氧化损伤[J]. 中国生物化学与分子生物学报, 2013, 29(12): 1166-1171.
ZHOU Xue-Hong, LU Yao, CAO Ji-Xiang, LI Shu-Yan. Knockdown of NFκB p65 Sensitizes Cellular Oxidative Damage in HCT116 Human Colonic Epithelial Cells. Chinese Journal of Biochemistry and Molecular Biol, 2013, 29(12): 1166-1171.
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