Abstract：In senescent cells, gene expression is different from that in young cells and some of them can induce human fibroblast cell premature. Oncogene-induced senescence (OIS) is a robust and sustained anti-proliferative response brought about by oncogenic signaling. Evidence shows that mitogen-inducible gene 6 (MIG-6) is a tumor suppressor gene. MIG-6 acts as a negative feedback regulator of the ErbB RTK pathway， and inhibits tumor cell proliferation. In this article we used human embryonic lung diploid fibroblast cell as a model to study the role of MIG-6 in the progress of cell senescence. Through Western blotting we found that the expression of MIG-6 in senescent cells increased. We constructed retroviral MIG-6 cDNA vector and transfected it into normal human fibroblast cells and analyzed MIG-6 expression in transfected cells. Then we examined the β-gal activity of HDF cells transfected with MIG-6 cDNA and found that the ratio of stained cells in transfected cells was much more than control cells, and the growth rate decreased. It was concluded that the over-expression of MIG-6 protein induced human embryonic lung diploid fibroblast cell premature.
