(Institute of Bioengineering, Jinan University, Guangzhou 510632, China; Key Laboratory of
Bioengineering Medicine of Guangdong Province, Guangzhou 510632, China)
Abstract：The promoter of human platelet-derived growth factor-β(PDGFR-β)gene tightly regulated during embryonic
development and in several physiological and pathological situations, was identified and characterized. Several deletions of the human PDGFR-β promoter were obtained by PCR from human genomic DNA
and then subcloned into pGL3-basic vector containing a luciferase reporter. The recombinant reporters were transiently transfected into the human umbilical vein endothelial cells (ECV304, HUVEC) for the
studies of the expression regulatory of this receptor. Two regions (-983~+53) and (+540~+1457) were identified to regulate human PDGFR-β promoter transcription by luciferase activity assays. The
former showed a positive regulatory role, while the latter was negative. The highly conserved regions of human, mouse and rat PDGFR-β core promoters were marked as A-box, B-box and C-box and were
found within these two regions. The B-box* and C-box* were observed to specifically bind nuclear factors by electrophoretic mobility shift assays.
张传宇;孙奋勇;时宿妹;马纪;谢秋玲. 人血小板源性生长因子受体β启动子的结构与功能分析[J]. 中国生物化学与分子生物学报, 2010, 26(04): 347-355.
ZHANGChuan-Yu;SUNFen-Yong;SHISu-Mei;MAJi;XIEQiu-Ling. Structural and Functional Analysis of Human PDGFR-β Promoter. Chinese Journal of Biochemistry and Molecular Biol, 2010, 26(04): 347-355.
