Abstract：Abstract Estrogen receptor-related receptor α（ERRα）is an orphan nuclear hormone receptor that shares significant homology with the estrogen receptors（ERs）and directly or indirectly modulate estrogen pathways ，which play important roles in pathogenesis of benign prostatic hyperplasia (BPH). To study the effects of prostate epithelial cell paracrine on the ERRα expression in prostate stromal cells (PrSCs), conditioned medium (CM) of BPH-1 (a benign prostate hyperplasia epithelial cell line) and DU-145 (a prostate cancer cell line) were collected and used to treat prostatic stromal cells. The expression of ERRα was determined by real-time RT-PCR and Western blotting, and the expressions of cyclooxygenase-2 (COX-2, prostaglandin synthase 2) of prostaglandin E2 (PGE2) was also examined by real-time RT-PCR and ELISA with or without the treatment of BPH-1 and DU-145 CMs. The CM of BPH-1 treated with NS-398 (a specific inhibitor of COX-2) was collected to analyze its effects on the ERRα expression. The results demonstrated that the CM from BPH-1, but not from DU-145, stimulated the mRNA expression of ERRα in PrSCs, which could be blocked by pretreat the CM producing cells with NS-398. The level of COX-2 mRNA, was significantly higher in BPH-1 than DU-145 CM treated, which correlated to the PGE2 secretion accordingly. The concentration of PGE2 in BPH-1 CM wit was reduced by NS-398, and PGE2 alone could stimulate ERRα expression in PrSCs. Our data suggested that BPH-1 CM could induce the ERRα expression by increasing the paracrine of PGE2, and promote the estrogen effect mediated by ERRα in PrSCs.
苗琳，石建党，周颖，杜小玲，吴荃，王克明，张琚. BPH-1通过分泌PGE2上调前列腺间质细胞ERRα的表达[J]. 中国生物化学与分子生物学报, 2008, 24(08): 742-747.
MIAOLin,SHIJian-Dang,ZHOUYing,DUXiao-Ling,WUQuan，WANGKe-Ming,ZHANGJu. Prostaglandin E2 Mediates the Upregulation of ERRα Expression with BPH-1 Condition Media in Prostatic Stromal Cells
. Chinese Journal of Biochemistry and Molecular Biol, 2008, 24(08): 742-747.
