Abstract：Osterix (Osx) is a zinc-finger-containing transcription factor which plays an important role in osteogenesis. Bone morphogenetic protein 2 (BMP2) may increase the transcription of Osx in osteoblast, but the mechanism remains unclear. The Osx transcription level in MC3T3-E1, C2C12 and C3H10T1/2 cell line, which possess osteoblast phenotypes, were analyzed by realtime quantitative RT-PCR. Following BMP2 stimulation, the Osxlevels were significantly upregulated, with the same induction kinetics as the important osteogenesis markers Col1a1 and osteocalcin. In addition, the introduction of a dominant negative Osx remarkably blocked the differentiation of C3H10T1/2 into osteocytes. The overexpression of Smad6, an inhibitor of BMP/Smad pathway, effectively diminished the BMP-induced upregulation of Osx in C3H10T1/2. The-1 254～+85 bp of Osxpromoter was cloned into a luciferase reporter plasmid, but response of BMP2 regulation to this region was not observed. Our results suggest that BMP2 upregulates Osx expression through the Smad pathway which is important for osteocyte differentiation.
潘秋辉,李益广,杨松海,马纪,谢在春,于永春,孙奋勇. 骨形态发生蛋白2通过Smad途径上调Osterix的表达[J]. 中国生物化学与分子生物学报, 2008, 24(01): 40-45.
PANQiu-Hui,LIYi-Guang,YANGSong-Hai,MAJi,XIEZai-Chun,YUYong-Chun,SUNFen-Yong. Bone Morphogenetic Protein 2 Upregulates Osterix Expression Through Smad Pathway . Chinese Journal of Biochemistry and Molecular Biol, 2008, 24(01): 40-45.
