HnRNP A/B蛋白D亚群的生物学功能

薛传静 陈维春 刘新光

中国生物化学与分子生物学报 ›› 2012, Vol. 28 ›› Issue (11) : 996-1004.

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中国生物化学与分子生物学报 ›› 2012, Vol. 28 ›› Issue (11) : 996-1004.
综述

HnRNP A/B蛋白D亚群的生物学功能

  • 薛传静1),2),刘新光1),2),3) ,陈维春1),3)*
作者信息 +

Biological Functions of D-subgroup Heterogeneous Nuclear Ribonucleoproteins A/B

  • XUE Chuan-Jing1),2),  LIU Xin-Guang1),2),3),  CHEN Wei-Chun1),3)*
Author information +
文章历史 +

摘要

核不均一核糖核蛋白(heterogeneous nuclear ribonucleoproteins, hnRNPs)是一类结合DNA和RNA的核蛋白,并能在核质间穿梭. A/B型hnRNPs(heterogeneous nuclear ribonucleoproteins,hnRNP A/B)是hnRNPs中研究最为清楚的一大类别,生物信息学分析hnRNP A/B可以区分成A和D两个亚群,已鉴定的D亚群主要成员包括hnRNP AB, D和DL. hnRNP A/B的D亚群均含有2个保守的RNA结合结构域(RNA binding domain, RBD)和1个Gly富集区(glycine-rich domain, GRD),成员间的主要区别在于N端和C端的长度和序列不同. D亚群与pre-mRNA和其它蛋白质结合成微粒系统,参与pre-mRNA的加工、稳定、核输出以及翻译过程. 此外,D亚群也能结合单链和双链DNA,参与转录起始和端粒的稳定. 因此,hnRNP A/B的 D亚群在各个阶段影响基因的表达,在神经系统发育、肿瘤的发生发展及衰老过程中发挥着多样性的功能.

Abstract

Heterogeneous nuclear ribonucleoproteins (hnRNPs) compose a kind of DNA and RNA binding proteins which shuttle between the nucleus and the cytoplasm. The A/B subfamily of hnRNPs is well characterized among all type hnRNPs and is classified into A-subgroup and D-subgroup by bioinformatics analyses. D-subgroup hnRNPs A/B include hnRNP AB, D and DL, all of which contain two conserved tandem RNA-binding domains (RBD) and a glycine-rich domain (GRD) near the carboxyl-terminus but differ in the length and the sequence of N-or C-terminus. D-subgroup of hnRNP A/B, packaged with pre-mRNA and other proteins into hnRNP particles, is involved in many processes such as premRNA splicing, stabilization, nuclear export, and protein translation. D-subgroup can also bind single-or double-strand DNA, suggesting potential roles in DNA replication and repair, transcription initiation and telomere maintenance. Taken together, these results indicate that member of hnRNPs A/B D-subgroup participate in every step of gene expression and play critical roles in neural development, tumorigenesis as well as progression, and aging processes.

关键词

核不均一核糖核蛋白 / mRNA加工 / 基因表达 / 衰老

Key words

heterogeneous nuclear ribonucleoproteins / mRNA splicing / gene expression / aging

引用本文

导出引用
薛传静 陈维春 刘新光. HnRNP A/B蛋白D亚群的生物学功能[J]. 中国生物化学与分子生物学报, 2012, 28(11): 996-1004
Biological Functions of D-subgroup Heterogeneous Nuclear Ribonucleoproteins A/B[J]. Chinese Journal of Biochemistry and Molecular Biology, 2012, 28(11): 996-1004
中图分类号: Q74    Q513   

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基金

国家自然科学基金(No.30971620,No.30900739),广东省自然科学基金(No.9252402301000002, No9152402301000007),东莞市科技计划项目(No2008108101062)和广东医学院建博科技创新团队项目(No.STIF201102)资助项目

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