建立高效液相色谱-蒸发光散射检测器分析神经酰胺的方法并进行了含量的测定. 色谱柱：ZORBZX Eclipse XDB-C18(4. 6 mm×250 mm, 5 μm)，洗脱方法：梯度洗脱，柱温：35 ℃，流动相：甲醇/水,流速：1 ml/min;检测器：蒸发光散射检测器,漂移管温度：40 ℃，氮气流速：1.5 L/min. 系统探讨了梯度洗脱的起始浓度、洗脱的时间和洗脱梯度的程序设置，最佳的梯度洗脱条件为5 min内，甲醇浓度从60% 线性增加为90%,从5 min到25 min,甲醇浓度线性增加为95%，在此条件下样品和标准品的分离色谱峰对称性较好.随后测定了各种样品中神经酰胺的含量，并进行了方法学验证，结果神经酰胺在0.2～2 μg之间线性关系良好, 最低检测限为0.01 mg/ml,R2=0.999 2;平均回收率为93.3%, RSD=1.65% (n=5).本法灵敏、方便、准确,重现性好,可用于魔芋神经酰胺类物质的分离及其含量的测定.
Abstract
analytical method using high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) has been developed for the determination of ceramide extracted from konjac. The simultaneous separation of ceramide was achieved on a ZORBZX Eclipse XDB-C18 column (4.6 mm×250 mm, 5 μm) by gradient elution with methanol-water as the mobile phase at the flow rate of 1 ml/min and the column temperature at 35 ℃. The parameters for ELSD were set by the drift tube temperature 40 ℃ and nitrogen flow-rate 1.5 L/min. After a serial of trials, the optimum elution process was by methanol concentration starting from 60% to 90% in 5min, and then to 95% in 20 min. Under the optimized conditions, the logarithmic linear curve can be obtained from 0.2 to 2 μg with R2 of 0. 999 2 and the detection limit (S/N=3) could reach 0.01 mg/ml. The average recovery was 93.3% with RSD =1.65% (n=5). The results demonstrate that this method provides good reproducibility and sensitivity for the quantification and it is simple, reliable and suitable for the quality control of ceramide extracted from konjac.
关键词
神经酰胺 /
高效液相色谱法 /
蒸发光散射检测器
{{custom_keyword}} /
Key words
ceramide /
high-performance liquid chromatography /
evaporative light-scattering detector
{{custom_keyword}} /
中图分类号：
R917.720
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
［1］Hakornori S. Glycosphingolipids in cellular interaction，differentiation and oncogenesis ［J］. Annu Rev Biochem,1981,50:733-764
［2］Spiegel S, Merrill AH Jr. Sphingolipid metabolism and cell growth regulation［J］. FASEB J, 1996, 10(12): 1388-1397
［3］Hannun Y A.Functions of sphingolipids and sphingolipid breakdown products in cellular regulation［J］. Science,1989,243(4890):500-507
［4］Hannun Y A. The sphingomyelin cycle and the second messenger function of ceramide［J］.J Biol Chem,1994, 269(5):3125-3128
［5］Hannun Y A. Function of ceramide in coordinating cellular responses to stress ［J］.Science,1996, 274(5294)：1855-1859
［6］Diatlovitskaia E V.Sphingoliplds and malignant growth［J］. Biokhimila(RUS),1995,60(6):843-850
［7］谢建平,张敏莲,刘铮. 神经酰胺及其分析与分离技术研究进展［J］.精细化工(Xie Jian-Ping,Zhang Min-Lian,Liu Zheng. Ceramides and recent developments in their isolation and analysis［J］.Fine Chem), 2002,19(7):31-35
［8］池静端,刘爱茹,徐礼燊. HPLC法测定银杏叶中三种内酯成分［ J ］.药物分析杂志(Chi Jing-Duan, Liu Ai-Ru, Xu Li-Xin. Determination of three ginkgolides in ginkgo biloba leaves by HPLC［J］.Chin J Pharm anal),1998, 18(6) : 367-369
［9］Van Beek T A, Scheeren HA, RantioT. Determination of ginkgolides and bilobalide in Ginkgo biloba leaves and phytopharmaceuticals ［ J ］. J Chromatogr, 1991, 543:375-
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
PDF(324 KB)
