过表达外源M-CSF促进MCF7细胞增殖

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中国生物化学与分子生物学报 ›› 2009, Vol. 25 ›› Issue (02) : 132-136.

研究论文

过表达外源M-CSF促进MCF7细胞增殖

涂剑^1）; 姚志红^1）; 龙治峰^2）;朱炳阳^1）; 唐圣
松^1）*

作者信息 +

Overexpression of M-CSF Promotes Proliferation of MCF7 Cells

TU Jian^1);YAO Zhi-Hong^1);LONG Zhi-Feng^2);ZHU Bing-Yang
¹⁾;TANG Sheng-Song^{1) *}

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文章历史 +

摘要

为探讨过表达外源巨噬细胞集落刺激因子(M-CSF)对细胞增殖的影响，将重组载体
pCMV/cyto/myc-M-CSF转染MCF7细胞、G418筛选，RT-PCR、Western 印迹和免疫荧光鉴定M-CSF的mRNA表达、蛋白表达及定位，通过计算细胞倍增时间、MTT法及反义寡核苷酸抑制实验分析M-CSF对细胞增殖的影响. 结果表明，转染M-CSF的MCF7细胞过表达M-CSF-mRNA及蛋白，并且定位表达于细胞质；与转染空载体及未转染M-CSF组细胞比较，转染M-CSF的MCF7细胞倍增时间明显缩短、增殖能力显著增强，M-CSF的特异性反义寡核苷酸能抑制转染M-CSF的MCF7细胞的增殖，且抑制率随着反义寡核苷酸浓度的增高而增强；以上结果提示，胞质过表达M-CSF可促进MCF7细胞的增殖.

Abstract

To explore the effect of macrophage colony stimulating factor (M-CSF)
on the cell proliferation, pCMV/cyto/myc-M-CSF vectors were transfected into
MCF7 cells using liposomes and screened with G418. RT-PCR, Western blot and immunofluorescence assays were performed to verify the M-CSF mRNA or protein expressions and localization. The results indicated that a high level of M-CSF expression in the cytoplasm was detected which promoted cell proliferation as shown by cell doubling time, MTT assays in M-CSFtransfected MCF7 cells. In addition, the proliferation of MCF7 cells transfected with M-CSF-specific antisense oligonucleotides was significantly inhibited than those of pCMV/cyto/myc transfected or untransfected MCF7 cells. We conclude that the overexpression of MCSF promotes the proliferation of MCF7 cells.

导出引用

涂剑;姚志红;龙治峰;朱炳阳;唐圣松. 过表达外源M-CSF促进MCF7细胞增殖[J]. 中国生物化学与分子生物学报, 2009, 25(02): 132-136

TUJian;YAOZhi-Hong;LONGZhi-Feng;ZHUBing-Yang;TANGSheng-Song.

Overexpression of M-CSF Promotes Proliferation of MCF7 Cells

[J]. Chinese Journal of Biochemistry and Molecular Biology, 2009, 25(02): 132-136

中图分类号： Q28

参考文献

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收稿日期	修回日期	出版日期
2008-08-04	1900-01-01	2009-02-20
发布日期
2009-02-20

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