灵芝(Ganoderma lucidum)漆酶基因的克隆及其序列分析

张银波,江木兰,胡小加,张桂敏,马立新

中国生物化学与分子生物学报 ›› 2005, Vol. 21 ›› Issue (05) : 699-703.

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中国生物化学与分子生物学报 ›› 2005, Vol. 21 ›› Issue (05) : 699-703.
论文

灵芝(Ganoderma lucidum)漆酶基因的克隆及其序列分析

  • 张银波,江木兰,胡小加,张桂敏,马立新
作者信息 +

Cloning and Sequence Analysis of a Laccase Gene from Ganoderma lucidum

  • ZHANG Yin-Bo 1),2) , JIANG Mu-Lan 2) , HU Xiao-Jia 2) , ZHANG Gui-Min 1) , MA Li-Xin 1)*
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Abstract

The oxidizing enzyme laccase produced by many fungi is generally considered to be active in the biodegradation of lignin, a major plant cell wall component highly resistant to microbial attack. Using degenerate primers and molecular techniques such as genome-walking and RT-PCR, a laccase gene and its corresponding cDNA were cloned from Ganoderma lucidum. The coding region of the genomic laccase sequence, which is preceded by the eukaryotic promoter elements TATA and CAAT, spans more than 2107 bp. The corresponding laccase cDNA was identical to the genomic sequence except for 9 introns that were 51-78 bp long. Sequence analysis indicated that the G. lucidum lccGl product has 10 potential N-glycosylation sites (Asn-Xxx-Ser/Thr) and four conserved fungi laccase amino acids signature sequences. The G. lucidum laccase sequence is most similar to the sequence of the laccase from Flammulina velutipes (similarity 73%).

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张银波,江木兰,胡小加,张桂敏,马立新. 灵芝(Ganoderma lucidum)漆酶基因的克隆及其序列分析[J]. 中国生物化学与分子生物学报, 2005, 21(05): 699-703
ZHANG Yin-Bo 1),2) , JIANG Mu-Lan 2) , HU Xiao-Jia 2) , ZHANG Gui-Min 1) , MA Li-Xin 1)*. Cloning and Sequence Analysis of a Laccase Gene from Ganoderma lucidum[J]. Chinese Journal of Biochemistry and Molecular Biology, 2005, 21(05): 699-703

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