采用过氧化氢作用于人脐静脉内皮细胞 ,成功地制作了氧化应激损伤的细胞模型 ,将培养的细胞分为 3组 :正常对照组 ,损伤组 (给予过氧化氢 )和血红素组 (同损伤组的制备 ,预先加入血红素 ) .观察各组细胞在光、电镜下形态学和蛋白质电泳的改变 ,用MTT法检测了各组细胞的生长状态 .结果表明 :在血红素组 ,细胞生长状态、MTT水平和蛋白质电泳结果与损伤组相比有明显好转且差异显著 ,证明血红素对人脐静脉内皮细胞氧化应激损伤有明显的保护作用 .
Abstract
The cell model of oxidative\|stress damage was made successfully by hydrogen peroxide in human umbilicus vein endothelial cells.The cultured cells were divided into three groups:control group,damage group in whicah H\-2O\-2 was given,and hemin group in which hemin was added previously.The cell growth conditions in the three groups were observed under the light microscope and the electron microscope.The cross\|linking proteins were analyzed by SDS\|PAGE and the surviving cells counted by MTT.There were significant differences between damage group and hemin group in cell growth condition,MTT and SDS\|PAGE pattern.The results demonstrated that hemin could protect the human umbilicus vein endothelial cells against oxidative\|stress damage.
关键词
血红素 /
H2O2 /
人脐静脉内皮细胞 /
氧化应激损伤 /
抗氧化保护
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Key words
hemin /
hydrogen peroxide /
human umbilicus vein endothelial cells /
oxidative\|stress damage /
protection against oxidative\|stress damage
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参考文献
1 薛雁,任晓君,张鲲,牟颖,刘俊秋,曹鹏,郭灿辉,罗贵民,沈家骢.具有谷胱甘肽过氧化物酶活性的小分子模拟物2TeCD保护线粒体抗氧化损伤.中国生物化学与分子生物学报(XueYan,RenXiao jun,ZhangKun,MuYing,LiuJun qiu,CaoPeng,GuoCan hui,LuoGui min,ShenJia cong.ProtectionofmitochondriaagainstoxidativedamagebytheGPXmimic2TeCD .ChinJBiochemMolBiol),2002,18(4):506～510
2 刘长振,王爱民,谢振华,聂丹瑶,马春,杨歌德,贺雨虹.肌肽对PC12细胞氧化应激损伤的保护作用.基础医学与临床(LiuChang zhen,WangAi min,XieZhen hua,NieDan yao,MaChun,YangGe de,HeYu hong.Protectiveeffectofcarnosineagainstoxidative stressdamageinPC 12cell.BasicMedSciClin),2001,21:551～553
3 HipkessAR ,WorthingtonVC ,HimsworthDTJ,HerwingW .Protectiveeffectofcarnosineagainstproteinmodificationmediatedbymalondialdehydeandhypochlorite.BiochimBiophysActa,1998,1380:46～54
4 王传清,袁弥满.氯化高铁血红素对正常及再生障碍性贫血小鼠红系祖细胞的体内诱导作用.中华血液学杂志(WangChuan qing,YuanMi man.Invivoinductiveeffectofheminontheerythroidprogenitorofthenormalandaplasticanemiamice.ClinJHematol),1998,19:507～509
5 HansenMB ,NielsenSE ,BergK .Re examinationandfurtherdevelopmentofapreciseandrapiddyemethodformeasuringcellgrowth cellkill.JImmunolmethods,1989,119:203～210
6 StadtmanER .Proteinoxidationandaging.Science,1992,257:1220～1224
7 OgawaK .Hememetabolisminstressresponse.NipponEiseigakuZasshi,2002,56:615～621
8 PetracheI ,OtterbeinLE ,AlamJ,WiegandGW ,ChoiAM .Hemeoxygenase1inhibitsTNF α inducedapoptosisinculturedfibroblasts.AmJPhysiol,LungCellMolPhysiol,2000,278:L312～319
9 StockerR ,YamamotoY ,McDonaghAF ,GlazerAN ,AmesBN .Bilirubinisanantioxidantofpossiblephysiologicalimportance.Science,1987,27(235):1043～1046
10 JornotL ,PetersenH ,JunodAF .Hydrogenperoxide inducedDNAdamageisindependentofnuclearcalciumbutdependentonredox activeions.BiochemJ ,1998,335:85～94
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