大豆Kunitz型胰蛋白酶抑制剂新类型Tid的全序列分析

忻骅,曹凯鸣,谢可方,顾其敏

中国生物化学与分子生物学报 ›› 1999, Vol. 15 ›› Issue (04) : 671-673.

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PDF(291 KB)
中国生物化学与分子生物学报 ›› 1999, Vol. 15 ›› Issue (04) : 671-673.
论文

大豆Kunitz型胰蛋白酶抑制剂新类型Tid的全序列分析

  • 忻骅,曹凯鸣,谢可方,顾其敏
作者信息 +

The Amino Acid Sequence of Kunitz type Soybean Trypsin Inhibitor, Ti d,Derived from Its Nucleotide Sequence and Its Comparison with Ti a

  • XIN Hua,CAO Kaiming,XIE Kefang,GU Qimin
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文章历史 +

Abstract

It was widely thought that 3 variants of Kunitz type trypsin inhibitor(SBTi A 2) existed in soybean seed storage protein.Three of codominant alleles Ti a,Ti b and Ti c were identified to decode these SBTi A 2 inhibitors and the amino acid sequences of them were determined,of which one or more different amino acid were found.Ti d was a new variant allele of SBTi A 2 discovered after analyzing more than 15 000 samples of soybean seed in China.In order to study the structure features of Ti d protein,the amino acid sequence of this protein was deduced from its coding region which was amplified by PCR from soybean genomic DNA and sequenced.By comparison with Ti a protein,two different amino acid residue were found between Ti a and Ti d proteins.One was an extra Ala inserted in the signal peptide of Ti d,with 8 residues from N terminal,and the other existed in the mature protein,with Glu 69 in Ti a protein turned into Lys 69 in Ti d protein.The amino acid sequence of Ti d mature protein was also different from those of Ti a and Ti b.

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忻骅,曹凯鸣,谢可方,顾其敏. 大豆Kunitz型胰蛋白酶抑制剂新类型Tid的全序列分析[J]. 中国生物化学与分子生物学报, 1999, 15(04): 671-673
XIN Hua,CAO Kaiming,XIE Kefang,GU Qimin. The Amino Acid Sequence of Kunitz type Soybean Trypsin Inhibitor, Ti d,Derived from Its Nucleotide Sequence and Its Comparison with Ti a[J]. Chinese Journal of Biochemistry and Molecular Biology, 1999, 15(04): 671-673

参考文献

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