以人肺癌细胞 Ｐ Ｇ 和人胃癌细胞 Ｂ Ｇ Ｃ８２３ 作为研究对象，利用 Ｍ Ｔ Ｔ 测定、３ Ｈ Ｔｄ Ｒ 参入、流式细胞术、软琼脂培养、 Ｎｏｒｔｈｅｒｎ ｂｌｏｔ、 Ｗ ｓｔｅｒｎ ｂｌｏｔ 等实验方法，观察了稀土化合物氯化亚鈰（ Ｃｅ Ｃｌ３）抑癌作用．结果表明， Ｃｅ Ｃｌ３ 浓度为 ００５ ｍ ｍ ｏｌ／ Ｌ，０１ ｍ ｍ ｏｌ／ Ｌ，０５ ｍ ｍ ｏｌ／ Ｌ和 １ ｍ ｍ ｏｌ／ Ｌ可抑制 Ｐ Ｇ 细胞的增殖；浓度为 ０５ ｍ ｍ ｏｌ／ Ｌ和 １ ｍ ｍ ｏｌ／ Ｌ可抑制 Ｐ Ｇ 细胞 Ｄ Ｎ Ａ 的合成，其 Ｇ１ 期细胞比例增加而 Ｓ期细胞比例减少，在软琼脂中的生长能力降低，原癌基因 ｃｍ ｙｃ 和 ｃｒａｓ 表达降低，ｐ１６ 蛋白质表达降低．而同样浓度的 Ｃｅ Ｃｌ３ 对 Ｂ Ｇ Ｃ８２３ 细胞和正常细胞 ２ Ｂ Ｓ未见影响．提示：稀土化合物抑制肺癌细胞 Ｐ Ｇ 的增殖以及降低其恶性度的作用机制可能与一些增殖相关的原癌基因的表达和细胞周期的调控有关，其确切的机理还需进一步的研究．
Abstract
The effect of CeCl 3 on human lung cancer cells PG,human gastric carcinoma cells BGC 823 and human diploid fibroblasts 2BS were studied by using MTT assay, 3H TdR incorporation,flow cytometry,soft agar culture,Northern blot and Western blot.The results indicated that CeCl 3 inhibited PG cells proliferation and 3H TdR incorporation;decreased the proportion of PG cells in S phase,while increased the proportion of PG cells in G1 phase:inhibited the growth of PG cells in soft agar and the expression of proto oncogenes c myc,c ras and p16 protein in PG cells.But CeCl 3 had no effect on BGC 823 cells and 2BS cells.The mechanism,by which the rare earth compound CeCl 3 inhibited the proliferation of PG cells'and decreased its malignancy,may be concerned with some proto oncogenes relating to proliferation and protein expression and the regulation of cell cycle.But further studies are needed to learn the exact mechanism.
关键词
氯化亚鈰 /
细胞增殖 /
细胞周期 /
基因表达
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Key words
CeCl 3 /
Cell proliferation /
Cell cycle /
Gene expression
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