反义凝血酶受体基因的表达对人ASMC增殖的影响

张茜,孟宪敏,姜玉新,丁金凤,汤健,陈光慧

中国生物化学与分子生物学报 ›› 1999, Vol. 15 ›› Issue (03) : 484-487.

PDF(638 KB)
PDF(638 KB)
中国生物化学与分子生物学报 ›› 1999, Vol. 15 ›› Issue (03) : 484-487.
论文

反义凝血酶受体基因的表达对人ASMC增殖的影响

  • 张茜,孟宪敏,姜玉新,丁金凤,汤健,陈光慧
作者信息 +

Effect of Antisense Thrombin Receptor Gene on the Proliferation of Human Vascular Smooth Muscle Cells

  • ZHANG Qian, MENG Xianmin, JIANG Yuxin, DING Jinfeng
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文章历史 +

摘要

介入治疗后再狭窄的发生严重降低了该治疗手段的最终疗效.为探讨再狭窄的发生机理、寻找有效的预防手段,利用反义RNA技术构建了含有部分反义凝血酶受体(ATR)cDNA片段的真核表达质粒pcDNA3/ATR,并观察了其对培养的人主动脉平滑肌细胞(ASMC)增殖的影响.结果表明pcDNA3/ATR的瞬时转染即能显著抑制人ASMC的3H-TdR参入量,且该作用与导入的DNA量呈剂量依赖性.说明部分反义凝血酶受体基因的表达可以抑制ASMC的增殖

Abstract

Restenosis after angioplasty severely limits the final effect of this therapeutic technique. To study the fundamental mechanisms of restenosis and search for an effectively preventive approach, a eukaryotic expression vector (pcDNA3/ATR) containing a partial antisense thrombin receptor (ATR) gene was constructed by antisense RNA technique. The inhibition of the recombinant vector proliferation of cultured human aortic smooth muscle cells (ASMC) was studied after introducing pcDNA3/ATR into ASMC instantaneously. The results showed that 3 H TdR incorporation in the transfected human ASMC was inhibited with pcDNA3/ATR in a dose dependent manner. The expression of a partical antisense thrombin receptor gene could inhibit the proliferation of human ASMC.

关键词

再狭窄 / 反义凝血酶受体基因 / 主动脉平滑肌细胞

Key words

Restenosis / Antisense thrombin receptor gene / Aortic smooth muscle cell

引用本文

导出引用
张茜,孟宪敏,姜玉新,丁金凤,汤健,陈光慧. 反义凝血酶受体基因的表达对人ASMC增殖的影响[J]. 中国生物化学与分子生物学报, 1999, 15(03): 484-487
ZHANG Qian, MENG Xianmin, JIANG Yuxin, DING Jinfeng. Effect of Antisense Thrombin Receptor Gene on the Proliferation of Human Vascular Smooth Muscle Cells[J]. Chinese Journal of Biochemistry and Molecular Biology, 1999, 15(03): 484-487

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