Monoclonal antibody (McAb) PD4 against gastric cancer was absorbed on Sepharose CL-4B-Pro A and then treated with crosslinking agent dimethyl pimelimi date dihydrochloride to form affinity medieum Sepharose CL-4B-Pro A-PD4 (columnⅠ). Column I had obvious advantages in Purifing anti-PD4-idiotypic antibody (αIdAb) . over the Sepharoae CL-4B-PD4 column (column Ⅱ).The binding capacity of columnⅠ with αIdAb was 1.78-1.94 times of that of column n .And the αIdAb purified with column Ⅰ had higher ability to inhibit in combination of PD4 with target cells than that of column Ⅱ . When the ratio of aldAb from column I to McAb PD4 was 1:1 and 4:1, the inhibition could reach 50% and 100% respectively, whereas for aldAb purified with column Ⅱ, the ratio of 2:1 and 8:1 was needed to exhibit same inhibition effects. Because of the protein A may combine with the Fc fragments of McAb PD4 to expose the PD4 Fab fragmets, therefore it is possible to provide PD4 with more opportunity for combining with aldAb.
Shou, Cheng-chao Dong, Zhi-wei Liu, Pei-nan.
The Purification of Anti-Idiotypic Antibody With Monoclonal Antibody Attached to Agarose through Protein A[J]. Chinese Journal of Biochemistry and Molecular Biology, 1990, 6(01): 61-65
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