Cibacron blue T_3GA与溴化氰活化的Sepharose 4B偶联后,产生一种能有效地分离有机磷水解酶的吸附剂。用0.15mol/L MgCl_2溶液从黄杆菌P3—2细胞抽提出的粗酶液通过柱层析分离,即可得到纯化8倍、酶活性回收率为269.4％的纯酶制品。该酶制品用凝胶电泳测是均一的。

The dye cibacron blue T3GA was coupled to cyanogen bromide activated sepha-rose 4B and the resulting affinity matrix was shown to be highly afficient for the purification of hydrolase of organophosphorus pesticides.A crude enzyme preparation from the bacterium flavobacterium SP P3-2 was extracted with 0.15mol/L MgCl2 at pH7.0. The crude enzyme was purified 8 fold by using this chromatography proce-dure, and a recovery of 269.4 per cent of the enzyme activity was obtained. The purified enzymes were shown to be homogeneous on poly acrylamide gel electropho-resis.