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| Analysis of Differential Expression Proteins of U937 Infected with Isoniazid-resistant MTB by Two-dimensional Electrophoresis |
| LIULi-Rong1),YUEJun1）,2),WANGHong-Hai1 |
1)State Key Laboratory of Genetic Engineering, Institute of Genetics,
School of Life Sciences, Fudan University, Shanghai 200433，China;
2)Shanghai Pulmonary Hospital, Shanghai 200433, China)
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Abstract Two-dimensional electrophoresis (2-DE) were employed to compare the global protein patterns between human macrophage cell line U937 infected with Mycobacterium Tuberculosis (MTB) isoniazid(INH)resistant strains and INHsusceptible strains, 86 protein spots were expressed differentially. Eight differential expression protein spots were measured by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). The data obtained from peptide mass fingerprinting were used for protein database search. Two protein spots that differentially express in U937 infected with MTB INH-resistant strains were identified as stem cell growth factor, MHC class Ⅰantigen, respectively. Six protein spots that differentially express in U937 infected with MTB INH-susceptible strains were identified as tubulin-β4, signal transducer and activator of transcription 3 (STAT 3), elongation factor-2 kinase (eEF-2K), ring finger protein 29, zinc finger protein 193 and SNARE Vti1a-β protein. These data provide insight into the changed global protein patterns of the U937 infected with MTB INH-resistant strains and INH-susceptible strains, and interpret the lower virulence, lower pathogenicity, lower infectivity, and longer incubation period that infected with INH-resistant strains in clinical cases. These results may contribute to the further research of new vaccines against INH-resistant strains. Key words〓
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Received: 14 June 2005
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Corresponding Authors:
WANG Hong-Hai1
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| [1] |
GUO Jia-Lin**, LI Zheng**, ZHANG Gai-Sheng*, WANG Shu-Ping, SONG Qi-Lu, LI Ying, MA Shou-Cai, NIU Na, WANG Jun-Wei. Optimization of Two-dimensional Electrophoresis System for the Study of Multi-ovary Character in Wheat[J]. Chinese Journal of Biochemistry and Molecular Biol, 2015, 31(9): 999-1007. |
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