[1]  Tallen G, Riabowol K. Keep-ING balance: tumor suppression by epigenetic regulation[J]. FEBS Lett, 2014, 588(16): 2728-2742
[2]  Tomczak K, Czerwinska P, Wiznerowicz M. The Cancer Genome Atlas (TCGA): an immeasurable source of knowledge[J]. Contemp Oncol (Pozn), 2015, 19(1A): A68-77
[3]  Zhang Z, Li H, Jiang S , et al. A survey and evaluation of Web-based tools/databases for variant analysis of TCGA data[J]. Brief B ioinform, 2019, 20(4): 1524-1541
[4]  Vogelstein B, Papadopoulos N, Velculescu VE , et al. Cancer genome landscapes[J]. Science, 2013, 339 (6127): 1546-1558
[5]  Yamagiwa K. Experimentelle studie uber die pathogenese der epithelialgeschwulste[J]. Mitt Med Fad Tokio, 1915, 15: 295-344
[6]  Loeb LA, Harris CC. Advances in Chemical Carcinogenesis: A Historical Review and Prospective[J]. Cancer Res, 2008, 68(17): 6863-6872
[7]  Westcott PM, Halliwill KD, To MD , et al. The mutational landscapes of genetic and chemical models of Kras-driven lung cancer[J]. Nature, 2015, 517(7535): 489-492
[8]  McCreery MQ, Halliwill KD, Chin D , et al. Evolution of metastasis revealed by mutational landscapes of chemically induced skin cancers[J]. Nat Med, 2015, 21(12): 1514-1520
[9]  Connor F, Rayner TF, Aitken SJ , et al. Mutational landscape of a chemically-induced mouse model of liver cancer[J]. J Hepatol, 2018, 69(4): 840-850
[10]  May M. Cancer research with a human touch[J]. Nature, 2018, 556(7700): 259-261
[11]  Galuschka C, Proynova R, Roth B , et al. Models in Translational Oncology:  A Public Resource Database for Preclinical Cancer Research[J]. Cancer Res, 2017, 77(10): 2557-2563
[12]  de Ruiter JR, Wessels LFA, Jonkers J. Mouse models in the era of large human tumour sequencing studies [J]. Open Biol, 2018, 8(8): 1-16
[13]  Adams JM, Harris AW, Pinkert CA , et al. The c-myc oncogene driven by immunoglobulin enhancers induces lymphoid malignancy in transgenic mice[J]. Nature, 1985, 318(6046): 533-538
[14]  Sinn E, Muller W, Pattengale P , et al. Coexpression of MMTV/v-Ha-ras and MMTV/c-myc genes in transgenic mice: synergistic action of oncogenes in vivo[J]. Cell, 1987, 49(4): 465-475
[15]  Molyneux G, Geyer FC, Magnay FA, et al. BRCA1 basal-like breast cancers originate from luminal epithelial progenitors and not from basal stem cells[J]. Cell Stem Cell, 2010, 7(3): 403-417
[16]  Dow LE, Fisher J, O'Rourke KP , et al. Inducible in vivo genome editing with CRISPR-Cas9[J]. Nat Biotechnol, 2015, 33(4): 390-394
[17]  Beer PA, Eaves CJ. Modeling Normal and Disordered Human Hematopoiesis[J]. Trends Cancer, 2015, 1(3): 199-210
[18]  Fridman AL, Tainsky MA. Critical pathways in cellular senescence and immortalization revealed by gene expression profiling[J]. Oncogene, 2008, 27(46): 5975-5987
[19]  Coursen JD, Bennett WP, Gollahon L , et al. Genomic Instability and Telomerase Activity in Human Bronchial Epithelial Cells during Immortalization by Human Papillomavirus-16 E6 and E7 Genes[J]. Exp Cell Res, 1997, 235(1): 245-253
[20]  Reddel RR, Ke Y, Gerwin BI , et al. Transformation of Human Bronchial Epithelial Cells by Infection with SV40 or Adenovirus-12 SV40 Hybrid Virus, or Transfection via Strontium Phosphate Coprecipitation with a Plasmid Containing SV40 Early Region Genes[J]. Cancer Res, 1988, 48(7): 1904-1909
[21]  Garcia-Mesa Y, Jay TR, Checkley MA , et al. Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system[J]. J Neurovirol, 2017, 23(1): 47-66
[22]  Liu Y, Wang CH, Fan J , et al. Establishing a papillary craniopharyngioma cell line by SV40LT-mediated immortalization[J]. Pituitary, 2020. doi: 10.1007/s11102-020-01093-5
[23]  Tsai CC, Chen CL, Liu HC, et al. Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines[J]. J Biomed Sci, 2010, 17(1): 64
[24]  Beckenkamp LR, da Fontoura DMS, Korb VG, et al. Immortalization of Mesenchymal Stromal Cells by TERT Affects Adenosine Metabolism and Impairs their Immunosuppressive Capacity[J]. Stem Cell Rev Rep, 2020, 16(4): 776-791
[25]  Huang ZX, Mao XM, Lin DC, et al. Establishment and characterization of immortalized human eutopic endometrial stromal cells[J]. Am J Reprod Immunol, 2020, 83(3): e13213
[26]  De Filippis L, Ferrari D, Rota Nodari L, et al. Immortalization of human neural stem cells with the c-myc mutant T58A[J]. PLoS One, 2008, 3(10):e 3310
[27]  De Filippis L, Lamorte G, Snyder EY, et al. A novel, immortal, and multipotent human neural stem cell line generating functional neurons and oligodendrocytes[J]. Stem Cells, 2007, 25(9): 2312-2321
[28]  Toouli CD, Huschtscha LI, Neumann AA, et al. Comparison of human mammary epithelial cells immortalized by simian virus 40 T-Antigen or by the telomerase catalytic subunit[J]. Oncogene, 2002, 21(1): 128-139
[29]  Zhao Z, Fowle H, Valentine H, et al. Immortalization of human primary prostate epithelial cells via CRISPR inactivation of the CDKN2A locus and expression of telomerase[J]. Prostate Cancer Prostatic Dis, 2020. doi: 10.1038/s41391-020-00274-4
[30]  Nishiwaki M, Toyoda M, Oishi Y, et al. Immortalization of human hepatocytes from biliary atresia with CDK4R24C, cyclin D1, and TERT for cytochrome P450 induction testing[J]. Sci Rep, 2020, 10(1): 17503-
[31]  Lee KM, Nguyen C, Ulrich AB, et al. Immortalization with telomerase of the Nestin-positive cells of the human pancreas[J]. Biochem Biophys Res Commun, 2003, 301(4): 1038-1044
[32]  Chang Z, Ju H, Ling J, et al. Cooperativity of oncogenic K-ras and downregulated p16/INK4A in human pancreatic tumorigenesis[J]. PLoS One, 2014, 9(7): e101452
[33]  Ward TM, Iorns E, Liu X, et al. Truncated p110 ERBB2 induces mammary epithelial cell migration, invasion and orthotopic xenograft formation, and is associated with loss of phosphorylated STAT5[J]. Oncogene, 2013, 32(19): 2463-2474
[34]  Kavsan VM, Iershov AV, Balynska OV. Immortalized cells and one oncogene in malignant transformation:  old insights on new explanation[J]. BMC Cell Biol, 2011, 12:23
[35]  Goldstein AS, Huang J, Guo C, et al. Identification of a cell of origin for human prostate cancer[J]. Science, 2010, 329(5991): 568-571
[36]  Okada H, Tsubura A, Okamura A, et al. Keratin profiles in normal/hyperplastic prostates and prostate carcinoma[J]. Virchows Arch A Pathol Anat Histopathol, 1992, 421(2): 157-161
[37]  Parsons JK, Gage WR, Nelson WG, et al. p63 protein expression is rare in prostate adenocarcinoma:  implications for cancer diagnosis and carcinogenesis[J]. Urology, 2001, 58(4): 619-624
[38]  Lee JK, Phillips JW, Smith BA, et al. N-Myc Drives Neuroendocrine Prostate Cancer Initiated from Human Prostate Epithelial Cells[J]. Cancer Cell, 2016, 29(4): 536-547
[39]  Park JW, Lee JK, Sheu KM, et al. Reprogramming normal human epithelial tissues to a common, lethal neuroendocrine cancer lineage[J]. Science, 2018, 362(6410): 91-95
[40]  Proia TA, Keller PJ, Gupta PB, et al. Genetic predisposition directs breast cancer phenotype by dictating progenitor cell fate[J]. Cell Stem Cell, 2011, 8(2): 149-163
[41]  Nguyen LV, Pellacani D, Lefort S, et al. Barcoding reveals complex clonal dynamics of de novo transformed human mammary cells[J]. Nature, 2015, 528(7581): 267-271.
[42]  Balani S, Nguyen LV, Eaves CJ. Modeling the process of human tumorigenesis[J]. Nat Commun, 2017, 8: 15422
[43]  Li M, Izpisua Belmonte JC. Organoids - Preclinical Models of Human Disease[J]. N Engl J Med, 2019, 380(6): 569-579
[44]  Zhou J, Su J, Fu X, et al. Microfluidic device for primary tumor spheroid isolation[J]. Exp Hematol Oncol, 2017, 6：22.doi:10.1186/s 40164-40171-0084-3
[45]  Sato T, Vries RG, Snippert HJ, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche[J]. Nature, 2009, 459(7244): 262-265
[46]  Barker N, van Es JH, Kuipers J, et al. Identification of stem cells in small intestine and colon by marker gene Lgr5[J]. Nature, 2007, 449(7165): 1003-1007
[47]  de Lau W, Barker N, Low TY, et al. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling[J]. Nature, 2011, 476(7360): 293-297
[48]  Matano M, Date S, Shimokawa M, et al. Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids[J]. Nat Med, 2015, 21(3): 256-262
[49]  Fumagalli A, Drost J, Suijkerbuijk SJ, et al. Genetic dissection of colorectal cancer progression by orthotopic transplantation of engineered cancer organoids[J]. Proc Natl Acad Sci U S A, 2017, 114(12): E2357-E2364
[50]  Drost J, van Jaarsveld RH, Ponsioen B, et al. Sequential cancer mutations in cultured human intestinal stem cells[J]. Nature, 2015, 521(7550): 43-47
[51]  Dekkers JF, Whittle JR, Vaillant F, et al. Modeling breast cancer using CRISPR/Cas9-mediated engineering of human breast organoids[J]. J Natl Cancer Inst, 2020, 112(5): 540-544
[52]  Sun L, Wang Y, Cen J, et al. Modelling liver cancer initiation with organoids derived from directly reprogrammed human hepatocytes[J]. Nat Cell Biol, 2019, 21(8): 1015-1026
[53]  Seino T, Kawasaki S, Shimokawa M, et al. Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression[J]. Cell Stem Cell, 2018, 22(3): 454-467
[54]  Proia TA, Keller PJ, Gupta PB, et al. Genetic predisposition directs breast cancer phenotype by dictating progenitor cell fate[J]. Cell Stem Cell, 2011, 8(2): 149-163
[55]  Jabs J, Zickgraf FM, Park J, et al. Screening drug effects in patient-derived cancer cells links organoid responses to genome alterations[J]. Mol Syst Biol, 2017, 13(11): 955
[56]  Drost J, Karthaus WR, Gao D, et al. Organoid culture systems for prostate epithelial and cancer tissue[J]. Nat Protoc, 2016, 11(2): 347-358
[57]  Drost J, Clevers H. Organoids in cancer research[J]. Nat Rev Cancer, 2018, 18(7): 407-418