Correlation between the Change of Intracellular Free Calcium Ion and Autophagy Induced by Quercetin in Hep G2 Cells

Chinese Journal of Biochemistry and Molecular Biology ›› 2013, Vol. 29 ›› Issue (3) : 264-269.

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Chinese Journal of Biochemistry and Molecular Biology ›› 2013, Vol. 29 ›› Issue (3) : 264-269.
Research Papers

Correlation between the Change of Intracellular Free Calcium Ion and Autophagy Induced by Quercetin in Hep G2 Cells

  • LUO Ya-Nan1), CUI Xiao-Dong 1), BAI Chong-Zhi2),LI Yu-Ying 1),WANG Zhuan-Hua 1)*
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Abstract

Quercetin has anticancer functions including induction of autophagy and inhibition of proliferation, but few reports were given on human hepatoma tumors and mechanism of autophagy of Hep G2. To understand the mechanism of cell autophagy induced by quercetin, we focused on the changes of intracellular free calcium ion concentration [Ca2+]i during autophagy of Hep G2 cells. The effect of quercetin on autophagy of Hep G2 cells was detected by laser scanning co-focal microscopy. [Ca2+]i and the level of autophagy were assessed by flow cytometry after Fluo-3 AM and Cyto-IDTM Green Detection Reagent staining. The results showed that the viabilities of Hep G2 cells were decreased and the autophagy was induced after treated with quercetin at concentration range of 0~50 μg/mL, while that of HL7702 cells were not changed significantly. Meanwhile, we also observed the effects of quercetin on intracellular free calcium ion and the relationship between autophagy and change of the [Ca2+]i in Hep G2 cells. It was found that [Ca2+]i in Hep G2 cells was increased significantly after treated with 50 μg/mL quercetin. However, autophagy was inhibited when incubated with an intracellular calcium chelator BAPTA-AM. These results revealed that [Ca2+]i elevation can promote autophagy while the reduction of [Ca2+]i may suppress cell autophagy. Autophagy induced by quercetin may be related to the change of intracellular free calcium ion.

Key words

quercetin / Hep G2 cells / intracellular free calcium ion / calcium chelator BAPTA-AM

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Correlation between the Change of Intracellular Free Calcium Ion and Autophagy Induced by Quercetin in Hep G2 Cells[J]. Chinese Journal of Biochemistry and Molecular Biology, 2013, 29(3): 264-269

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Funding

Supported by National Natural Science Foundation of China (No.30970611; No.31171659)

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