Analysis of SNP of PSCA Gene in Limited Gastric Cancer Cells Isolated by Laser Microdissection

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Chinese Journal of Biochemistry and Molecular Biology ›› 2012, Vol. 28 ›› Issue (6) : 567-573.

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Analysis of SNP of PSCA Gene in Limited Gastric Cancer Cells Isolated by Laser Microdissection

YANG Yi1)，　GUO Yan1),2)*，　ZHAO Xiao-Dong1)，　LIU Bing-Ya3)，　SHAO Zhi-Feng1),2)

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Abstract

With the applications of genome-wide association study (GWAS), more and more predisposing gene were identified in gastric cancer. The polymorphism detection of predisposing genes was applied in clinical diagnosis and research of gastric cancer. However, the detection was challenging with limited samples, such as limited gastric mucosal cells for early diagnosis. With laser microdissection and GenomePlex library whole genome amplification methods, single nucleotide polymorphism (SNP) of the prostate stem cell antigen (PSCA) in limited pure gastric cancer cells were studied. Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing method, rs2976392 and rs2976392, the predisposing mutations of PSCA gene, in microdissected gastric cancer tissue, were identified. The results showed that the sensitivity and reliability of predisposing mutation detection were greatly increased after pure gastric cancer cells isolation. Multi-gene SNP detection methods for limited sample developed can be applied to the genetic analysis of other types of limited sample. Whole genome amplification products can also be used for high-throughput DNA microarray and next-generation sequencing analysis.

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gastric cancer / limited sample / whole genome amplification / single nucleotide polymorphism (SNP) / prostate stem cell antigen (PSCA)

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YANG Yi, GUO Yan, ZHAO Xiao-Dong, LIU Bing-Ya, SHAO Zhi-Feng. Analysis of SNP of PSCA Gene in Limited Gastric Cancer Cells Isolated by Laser Microdissection[J]. Chinese Journal of Biochemistry and Molecular Biology, 2012, 28(6): 567-573

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Funding

Supported by the National Natural Science Foundation of China (No. 30900271), National Basic Research Program of China (973 Program,No. 2010CB529205) and State Key Laboratory of Oncogenes and Related Genes (No. 91-10-09)

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Received	Revised	Published
2012-01-17	2012-03-21	2012-06-20
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2012-06-20

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