Expression of Porcine SFRP4 and the Effect on Differentiation of Preadipocytes Via sp600125

GUAN Hua, ZHENG Jia-Meng, CHEN Zong-Zheng, SONG Zi-Yi, YANG Hao, YANG Gong-She

Chinese Journal of Biochemistry and Molecular Biology ›› 2012, Vol. 28 ›› Issue (5) : 455-463.

PDF(784 KB)
PDF(784 KB)
Chinese Journal of Biochemistry and Molecular Biology ›› 2012, Vol. 28 ›› Issue (5) : 455-463.
Reviews

Expression of Porcine SFRP4 and the Effect on Differentiation of Preadipocytes Via sp600125

  • GUAN Hua, ZHENG Jia-Meng, CHEN Zong-Zheng, SONG Zi-Yi, YANG Hao, YANG Gong-She*
Author information +
History +

Abstract

Secreted frizzledrelated protein 4(SFRP4) was the regulator of Wnt signaling pathway. The expression of SFRP4 in fat tissues of different genotype pigs and in different growth periods were explored by highthroughout sequencing (Solexa) and real-time quantity PCR (RT-qPCR). The effect on differentiation of preadipocyte and the expression level of SFRP4 were explored by inhibitor SP600125 in JNK signaling pathway. The results showed that expression level of SFRP4 was higher in fat tissues of obesetype than that in the lean-type. Expression level of SFRP4 in the porcine fat tissue and adipocyte was influenced by ages, and was higher in fat, but lower in other tissues. SP600125 promoted the adiocytes to differentiate and significantly increased expression levels of PPARγ, FABP4, ATGL, Perilipin, but the SFRP4 expression level was inhibited significantly. This research provides a new reference for screen the key genes in adipocyte differentiation.

Key words

secreted frizzled-related protein 4 / high-throughput sequencing / preadipocyte / inhibitor SP600125 / differentiation

Cite this article

Download Citations
GUAN Hua, ZHENG Jia-Meng, CHEN Zong-Zheng, SONG Zi-Yi, YANG Hao, YANG Gong-She. Expression of Porcine SFRP4 and the Effect on Differentiation of Preadipocytes Via sp600125[J]. Chinese Journal of Biochemistry and Molecular Biology, 2012, 28(5): 455-463

References

[1] Rattner A, Hsieh JC, Smallwood PM, et al. A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors [J].Proc Natl Acad Sci, 1997, 94(7): 2859-2863
[2] Suzuki H, Watkins DN, Jair KW, et al. Epigenetic inactivation of SFRP genes allows constitutive WNT signaling in colorectal cancer [J].Nat Genet, 2004, 36(4):417-422
[3] Cho HY, Choi HJ, Sun HJ, et al. Transgenic mice overexpressing secreted frizzled-related proteins (sFRP)4 under the control of serum amyloid P promoter exhibit low bone mass but did not result in disturbed phosphate homeostasis[J]. Bone, 2010, 47(2):263–271
[4] Stamper B D, Park S S, Beyer R P, et al. Differential Expression of Extracellular Matrix-Mediated Pathways in Single-Suture Craniosynostosis [J]. PLoS One, 2011, 6(10): e26557
[5] Zhang Y, Chen FQ, Sun YH et al. Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells[J]. J Exp Clin Cancer Res, 2011, 30(1): 98
[6] Ting AH, Suzuki H, Cope L, et al. A requirement for DICER to maintain full promoter CpG island hypermethylation in human cancer cells [J]. Cancer Res, 2008, 68(8): 2570–2575
[7] Easwaran HP, Van Neste L, Cope L, et al. Aberrant silencing of cancer-related genes by CpG hypermethylation occurs independently of their spatial organization in the nucleus [J]. Cancer Res, 2010, 70(20): 8015–8024
[8] Romaker D, Puetz M, Teschner S, et al. Increased expression of secreted frizzled-related protein 4 in polycystic kidneys [J] J Am Soc Nephrol, 2009, 20(1): 48–56
[9] Jacob F, Ukeqjini K ,Nixdorf S, et al. Loss of secreted frizzled-related protein 4 correlates with an aggressive phenotype and predicts poor outcome in ovarian cancer patients. [J].PLoS One, 2012, 7(2): e31885
[10] Moran A, Akcan Arikan A, Mastragelo MA. et al. Prevention of trauma and hemorrhagic shock-mediated liver apoptosis by activation of stat3alpha [J]. Int J Clin Exp Med, 2008, 1(3): 213–247
[11] Carmon K S, Loose D S. Development of a bioassay for detection of Wnt-binding affinities for individual frizzled receptors [J].Anal Biochem, 2010, 401(2): 288-294
[12] Tominaga S, Yamaguchi T, Takahashi S, et al. Negative regulation of adipogenesis from human mesenchymal stem cells by Jun N-terminal kinase [J]. Biochem Biophys Res Commun, 2005, 326 (2):499-504
[13] Fu L, Tang T, Miao Y, et al. Stimulation of osteogenic differentiation and inhibition of adipogenic differentiation in bone marrow stromal cells by alendronate via ERK and JNK activation [J].Bone, 2008, 43 (1): 40-47
[14] 杨公社,张浩卫,白亮,等. 猪—研究肥胖和糖尿病的理想模式动物[J].自然科学进展 (Yang GS, Zhang HW, Bai L, et al. Pig—A ideal study animal model of obesity and diabeties[J]. Prog Nat Sci ),2008, 18(5): 481−487
[15] 屈长青,张国华,陈粉粉,等.猪前体脂肪细胞的原代培养[J].农业生物技术学报(Qu Chang-Qing, Zhang Guo -Hua , Chen Fen-Fen, et al .Primary culture of porcine preadipocyte [J].J Agric Biotechnol),2005,13(5):649-653
[16] Ouchi N, Higuchi A, Ohashi K,et al. Sfrp5 is an anti-inflammatory adipokine that modulates metabolic dysfunction in obesity [J].Science,2010, 329(5990):454-457
[17] 李新建, 杨浩, 程佳,等.猪TCTP基因的表达规律及其对脂肪细胞分化的影响[J].中国生物化学与分子生物学报(Li Xin- Jian,Yang Hao,Cheng Jia,et al. Expression of porcine TCTP and its effect on differentiation of adipocytes [J].Chin J Biochem Mol Biol), 2011, 27(5): 444-451
[18] Sambrook J, Russell DW. Molecular Cloning, A Laboratory Manual, 3rd ed. New York: Cold Spring Harbor Laboratory Press,1989 (萨姆布鲁克J,拉塞尔DW 著,黄培堂,等译.分子克隆实验指南.第3版[M]. 北京: 科学出版社Beijing: Science Press, 2002)
[19] 高晓娟,吉红,常志光,等. PGC- 1 和 SREBP- 1c 在猪脂肪细胞分化过程中的相互作用[J].中国生物化学与分子生物(Gao Xiao-Juan,Ji Hong,Chang Zhi-Guang, et al. Interaction of PGC-1 and SREBP- 1c during porcine adipocyte  differentiation [J]. Chin J Biochem Mol Biol), 2011,27(6) :533-539
[20] Yam JW, Chan KW, Ngan ES, et al. Genomic structure, alternative splicing and tissue expression of rFrp/sFRP-4, the rat frizzled related protein gene [J]. Gene, 2005,357 (1):55–62
[21] White L, Suganthini G, Friis R,et al. Expression of secreted frizzled-related protein 4 in the primate placenta[J]. Reprod Biomed Online, 2009, 18(1):104–110
[22] Constantinou T, Baumann F, Lacher MD, et al. SFRP-4 abrogates Wnt-3a-induced beta-catenin and Akt/PKB signalling and reverses a Wnt-3a-imposed inhibition of in vitro mammary differentiation.[J]. J Mol Signal, 2008, 3(1):10–23
[23] Drake JM, Friis RR, Dharmarajan AM et al. The role of sFRP4, a secreted frizzled-related protein, in ovulation[J]. Apoptosis, 2003, 8(4):389–397
[24] Hsieh M, Mulders SM, Friis RR. Expression and localization of secreted frizzled-related protein-4 in the rodent ovary: evidence for selective up-regulation in luteinized granulosa cells [J]. Endocrinology, 2003, 144(10):4597–4606
[25] Fox S, Dharmarajan A .WNT signaling in malignant mesothelioma[J]. Front Biosci, 2006, 11(9):2106–2112
[26] Feng Han Q, Zhao W, Bentel J, et al. Expression of sFRP-4 and beta-catenin in human colorectal carcinoma[J]. Cancer Lett, 2006, 231(1):129–137
[27] Huang D, Yu B, Deng Y, et al. SFRP4 was overexpressed in colorectal carcinoma [J].J Cancer Res Clin Oncol, 2010, 136(3):395–401
[28] Drake J, Shearwood AM, White J et al. Expression of secreted frizzled-related protein 4 (SFRP4) in primary serous ovarian tumours[J].Eur J Gynaecol Oncol, 2009, 30(2):133-141
[29] Schumann H, Holtz J, Zerkowski HR, et al. Expression of secreted frizzled related proteins 3 and 4 in human ventricular myocardium correlates with apoptosis related gene expression [J]. Cardiovasc Res, 2000, 45(3):720-728
[30] Wang S, Krinks M, Lin K, et al. Frzb, a secreted protein expressed in the Spemann organizer, binds and inhibits Wnt-8 [J].Cell ,1997,88(3):757-766
[31] Park JR, Jung JW, Lee YS, et al. The roles of Wnt antagonists Dkk1 and SFRP4 during adipogenesis of human adipose tissue-derived mesenchymal stem cells [J].Cell Prolif, 2008, 41(6):859-874
[32] Suzuki S, Sembon S, Iwamoto M, et al. Identification of genes downregulated during differentiation of porcine mesenteric [J].J Anim Sci, 2008, 86(12):3367-3376
[33] Bennett CN, Ross SE, Longo KA, et al. Regulation of Wnt signaling during adipogenesis[J].Biol Chem,2002, 277(34) :30998-31004
[34] Hu E, Zhu Y, Fredrickson T, et al. Tissue restricted expression of two human Frzbs in preadipocytes and pancreas [J].      
Biochem Biophys Res Commun, 1998, 24(2):287-293
 

Funding

Supported by Genetically Modified Organisms Breeding Major Projects (No.2009ZX08009-157B)

PDF(784 KB)

904

Accesses

0

Citation

Detail

Sections
Recommended

/