Preparation and Application of Protein Microarray with Cell-free System

DI Cui-Xia, ZHANG Hong, YANG Li-Na, WANG Xiao-Hu

Chinese Journal of Biochemistry and Molecular Biology ›› 2012, Vol. 28 ›› Issue (1) : 28-34.

PDF(365 KB)
PDF(365 KB)
Chinese Journal of Biochemistry and Molecular Biology ›› 2012, Vol. 28 ›› Issue (1) : 28-34.
Reviews

Preparation and Application of Protein Microarray with Cell-free System

  • DI Cui-Xia 1), 2),  ZHANG Hong 1), 2)*,  YANG Li-Na3),  WANG Xiao-Hu4)
Author information +
History +

Abstract

Protein microarray is one of the key techniques in biotechnology and functional proteomics. The traditional method of producing protein is usually lengthy and expensive. The combination of cell-free expression system and protein microarray, avoids some complicated processes, such as the gene cloning, protein expression, purification and preservation, so that the whole process of cell-free protein microarray is quick, fast and efficient. This article reviews in detail the cell-free protein synthesis system and its classification, the latest progress on application of cell -free protein system in protein microarray, and explores the latest applications of cell-free protein microarray in proteomics research.

Key words

protein microarray / protein expression using cell-free systems / in vitro protein expression

Cite this article

Download Citations
DI Cui-Xia, ZHANG Hong, YANG Li-Na, WANG Xiao-Hu. Preparation and Application of Protein Microarray with Cell-free System[J]. Chinese Journal of Biochemistry and Molecular Biology, 2012, 28(1): 28-34

References

[1] Abbott A. And now for the proteome…[J].Nature, 2001, 409(6822): 747
[2] Fields S. Proteomics. Proteomics in genomeland[J].Science, 2001, 291(5507): 1221-1224
[3] Stoevesandt O, Taussig M J, He M. Protein microarrays: high-throughput tools for proteomics[J]. Expert Rev Proteomics, 2009, 6(2): 145-157
[4] 吕林莉, 刘必成.基于无细胞蛋白表达的蛋白质微阵列技术[J].生物医学工程杂志 (Lv Lin-Li, Liu Bi-Cheng. The cell-free protein synthesis-based protein microarray technology[J]. J Biomed Eng, 2010, 27(6):1937-1400
[5] 艾润娜, 赵晓航.自组装蛋白质芯片—功能蛋白质组学的新工具[J].生命科学 (Ai Run-Na, Zhao Xiao-Hang. Self-assembling protein microarray—a new tool for functional proteomics [J]. Chin Bull Life Sci, 2010, 22(10):985-990
[6] 张晓筱.蛋白质芯片——蛋白质高表达及固定技术研究进展[J].贵州师范大学学报(自然科学版)(Zhang Xiao-Xiao. Review of protein chip technology: recent development in protein high throughput expression and attachment [J]. J Guizhou Norm Univ(Nat Sci), 2007,25(1):113-118
[7] Stevens R C. Design of high-throughput methods of protein production for structural biology[J].Structure, 2000, 8(9): R177- 185
[8] Ramachandran N, Hainsworth E, Bhullar B, et al. Self-assembling protein microarrays[J]. Science, 2004, 305(5680): 86-90
[9] He M, Taussig M J. Single step generation of protein arrays from DNA by cell-free expression and in situ immobilisation (PISA method)[J]. Nucleic Acids Res, 2001, 29(15): E73
[10] Angenendt P, Kreutzberger J, Glokler J, et al. Generation of high density protein microarrays by cell-free in situ expression of unpurified PCR products[J]. Mol Cell Proteomics, 2006, 5(9): 1658-1666
[11] Jackson A M, Boutell J, Cooley N, et al. Cell-free protein synthesis for proteomics[J].Brief Funct Genomic Proteomic, 2004, 2(4): 308-319
[12] Zubay G. In vitro synthesis of protein in microbial systems[J]. Annu Rev Genet, 1973, 7: 267-287
[13] Spirin A S, Baranov V I, Ryabova L A, et al. A continuous cell-free translation system capable of producing polypeptides in high yield[J]. Science, 1988, 242(4882): 1162-1164
[14] 章杰, 刘琼明, 徐丹科, 等. 无细胞系统原位制备蛋白质芯片及其应用[J].生物化学与生物物理进展(Zhang Jie, Liu Qiong-Ming, Xu Dan-Ke, et al. In situ fabrication and application of protein microarray with cell-free system [J]. Prog Biochem Biophys), 2009,36(4):391-397
[15] Nirenberg M W, Matthaei J H. The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides[J].Proc Natl Acad Sci U S A,1961, 47: 1588-1602
[16] Endo Y, Sawasaki T. Cell-free expression systems for eukaryotic protein production[J].Curr Opin Biotechnol, 2006, 17(4): 373-380
[17] 王润华, 沈培奋, 李钰云,等.一种简单快速微量的无细胞蛋白合成系统[J].中国生物化学与分子生物学报(Wang Run-Hua, Shen Pei-Fen, Li Yu-Yun, et al. A rapid and simplified microarray for measurement of protein synthesis in rabbit reticulocyte lysates[J].Chin J Biochem Mol Biol), 1995, 11(4):430-433
[18] 王润华, 郑硕, 陈兴,等. 核糖体单链失活蛋白在无细胞体系中的活性[J].中国生物化学与分子生物学报(Wang Run-Hua, Zheng Shuo, Chen Xing, et al. Inhibition of protein synthesis in cell-free system by single chain ribosome-inactivating proteins [J].Chin J Biochem Mol Biol), 1992, 8(4):395-399
[19] Roberts B E, Paterson B M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ [J]. Proc Natl Acad Sci U S A, 1973, 70(8): 2330-2334
[20] Takai K, Endo Y. The cell-free protein synthesis system from wheat germ [J].Methods Mol Biol, 2010, 607: 23-30
[21] 王景林,高培基. 微紫青霉CBHⅠ基因(Cbh1)在体外无细胞系统中的表达[J].中国生物化学与分子生物学报(Wang Jing-Lin, Gao Pei-Ji. In vitro expression of CBHⅠ gene(Cbh1) of Penicillium janthinellum in coupled transcription-translation system[J].Chin J Biochem Mol Biol),1999,15(6):885- 887
[22] 徐志男, 陈海琴, 汪家权,等.无细胞蛋白质合成系统的研究进展[J].中国生物化学与分子生物学报(Xu Zhi-Nan, Chen Hai-Qin, Wang Jia-Quan, et al. Advances in cell-free protein biosynthesis [J].Chin J Biochem Mol Biol), 2004,20(3):289-293
[23] Katzen F, Chang G, Kudlicki W. The past, present and future of cell-free protein synthesis[J]. Trends Biotechnol, 2005, 23(3): 150-160
[24] Matarraz S, Gonzalez-Gonzalez M, Jara M, et al. New technologies in cancer. Protein microarrays for biomarker discovery[J]. Clin Transl Oncol, 2011, 13(3): 156-161
[25] Ramachandran N, Srivastava S, Labaer J. Applications of protein microarrays for biomarker discovery[J]. Proteomics Clin Appl, 2008, 2(10-11): 1444-1459
[26] Ramachandran N, Raphael J V, Hainsworth E, et al. Next-generation high-density self-assembling functional protein arrays[J]. Nat Methods, 2008, 5(6): 535-538
[27] He M, Stoevesandt O, Palmer E A, et al. Printing protein arrays from DNA arrays[J]. Nat Methods, 2008, 5(2): 175-177
[28] Tao S C, Zhu H. Protein chip fabrication by capture of nascent polypeptides[J].Nat Biotechnol, 2006, 24(10): 1253-1254
[29] Murthy T V, Wu W, Qiu Q Q, et al. Bacterial cell-free system for high-throughput protein expression and a comparative analysis of Escherichia coli cell-free and whole cell expression systems[J]. Protein Expr Purif, 2004, 36(2): 217-225
[30] Davies D H, Molina D M, Wrammert J, et al. Proteome-wide analysis of the serological response to vaccinia and smallpox[J].Proteomics, 2007, 7(10): 1678-1686
[31] Eyles J E, Unal B, Hartley M G, et al. Immunodominant Francisella tularensis antigens identified using proteome microarray[J].Proteomics, 2007, 7(13): 2172-2183
[32] Wright C, Sibani S, Trudgian D, et al. Detection of multiple autoantibodies in patients with ankylosing spondylitis using nucleic acid programmable protein arrays[J]. Mol Cell Proteomics, 2010 [Epub ahead of print]
[33] Anderson K S, Sibani S, Wallstrom G, et al. Protein microarray signature of autoantibody biomarkers for the early detection of breast cancer[J]. J Proteome Res, 2011, 10(1): 85-96
[34] He M, Taussig M J. DiscernArray technology: a cell-free method for the generation of protein arrays from PCR DNA[J]. J Immunol Methods, 2003, 274(1-2): 265-270
[35] Di C X , Alhamdani M S, Christoph S, et al. Analysis of protein isoforms that are relevant to diagnostics and drug activity by means of antibody microarray; application to pancreatic cancer and lymphoma [C]. 3rd Annual meeting of NGFN-Plus and NGFN-Transfer in the Program of Medical Genome Research. Berlin, 2010


Funding

Supported by Major State Basic Research Development Program of China (973 Program, No.2010CB834202)and National Natural Science Foundation of China(No.10835011)

PDF(365 KB)

207

Accesses

0

Citation

Detail

Sections
Recommended

/