Knockdown of Human HPIP Gene Inhibits Cell Growth and Proliferation

XU Xiao-Jie, WANG Ling-Xue, FAN Zhong-Yi, Ding-Li-Hua, ZHANG Hao, Yang-Zhi-Hong, LI Jie-Zhi, YE Qi-Nong-*

Chinese Journal of Biochemistry and Molecular Biology ›› 2011, Vol. 27 ›› Issue (2) : 190-196.

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PDF(699 KB)
Chinese Journal of Biochemistry and Molecular Biology ›› 2011, Vol. 27 ›› Issue (2) : 190-196.
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Knockdown of Human HPIP Gene Inhibits Cell Growth and Proliferation

  • XU Xiao-Jie,WANG Ling-Xue,FAN Zhong-Yi,DING Li-Hua,ZHANG Hao,YANG Zhi-Hong,LI Jie-Zhi,YE Qi-Nong*
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Abstract

To study the biological function of hematopoietic PBX-interacting protein (HPIP) gene in the development of tumor,the eukaryotic expression vector of HPIP small interfering RNA(siRNA) was constructed and investigated its effect on cell growth and proliferation.HPIP siRNA was designed and constructed based on human HPIP cDNA sequence using pSilencer 2.1-U6 neo vector.The expression of HPIP was examined by Western blot and Real-time RT-PCR analysis.The effect of HPIP siRNA on cell growth and proliferation were analyzed by violet assay and FACS.HPIP siRNA was successfully cloned to the target vector,and was able to knockdown the expression level of HPIP gene.Violet assay and FACS analysis showed that down-regulation of HPIP obviously suppress cell growth and proliferation.Soft agar assay demonstrated HPIP siRNA decrease the anchorage-independent tumor cell growth.Taken together,the eukaryotic expression vector of HPIP siRNA obviously inhibits the expression of HPIP and suppresses cell growth and proliferation,which might serve as a therapeutic target in tumor treatment.

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XU Xiao-Jie, WANG Ling-Xue, FAN Zhong-Yi, Ding-Li-Hua, ZHANG Hao, Yang-Zhi-Hong, LI Jie-Zhi, YE Qi-Nong-*. Knockdown of Human HPIP Gene Inhibits Cell Growth and Proliferation[J]. Chinese Journal of Biochemistry and Molecular Biology, 2011, 27(2): 190-196

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Funding

 

Supported by Medical Research Project during the Eleventh-five Plan Period of PLA(No.06J021);National Science Foundation(No.30800205)and Major Research Program on Science and Technology of China(No.2007CB914603)
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