Regulation Of Erk1/2 Activity By Notch3 In Vascular Smooth Muscle Cells

ZHU Yun-Yi, LIU Xue-Xia, LI Ming-Gang, Nicholas Flavahan, HUANG Chang-Jiang, ZHU Jian-Hong

Chinese Journal of Biochemistry and Molecular Biology ›› 2011, Vol. 27 ›› Issue (11) : 1039-1043.

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Chinese Journal of Biochemistry and Molecular Biology ›› 2011, Vol. 27 ›› Issue (11) : 1039-1043.
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Regulation Of Erk1/2 Activity By Notch3 In Vascular Smooth Muscle Cells

  •  ZHU Yun-Yi1), LIU Xue-Xia2), LI Ming-Gang3), FLAVAHAN Nicholas4),
    HUANG Chang-Jiang5), ZHU Jian-Hong2)*
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Abstract

Activation of Erk1/2 affects a broad range of cellular functions in blood vessels. Notch3 is mainly expressed in vascular smooth muscle cells of arteries, and is necessary for arterial maturity during the development. In order to investigate the impact of Notch3 on Erk1/2 activation in vascular smooth muscle cells, Erk1/2 activation was induced by reactive oxygen species (ROS) and the level of Erk1/2 phosphorylation was analyzed by Western blotting after transfection with Notch3 siRNA or construct with Notch3 intracellular domain (NICD), or blocking Notch signaling by γsecretase inhibitor DAPT. Erk1/2 phosphorylation was decreased in vascular smooth muscle cells after siRNA knockout of Notch3 expression. Both Notch3 siRNA knockdown and DAPT inhibited ROSinduced Erk1/2 activation. No change in Erk1/2 phosphorylation was detected in NICD overexpressed vascular smooth muscle cells. However, NICD overexpression postponed the attenuation of ROSinduced Erk1/2 activation. In conclusion, Notch3 can regulate Erk1/2 activity and ROSinduced Erk1/2 activation in vascular smooth muscle cells.

Key words

Notch3 / Erk1/2 / vascular smooth muscle cells / reactive oxygen species (ROS)

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ZHU Yun-Yi, LIU Xue-Xia, LI Ming-Gang, Nicholas Flavahan, HUANG Chang-Jiang, ZHU Jian-Hong. Regulation Of Erk1/2 Activity By Notch3 In Vascular Smooth Muscle Cells[J]. Chinese Journal of Biochemistry and Molecular Biology, 2011, 27(11): 1039-1043

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Funding

Supported by Wenzhou Medical College Research Foundation (No.QTJ10024) and NIH (No.OH-8531,USA)

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