Overexpression of Fucosyltransferase Ⅳ Promotes Embryonic Cells to Adhere to Uterine Cells

LIU Shuai, FAN Li-Xue, YANG Xue-Song, YAN Qiu

Chinese Journal of Biochemistry and Molecular Biology ›› 2011, Vol. 27 ›› Issue (10) : 968-971.

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PDF(382 KB)
Chinese Journal of Biochemistry and Molecular Biology ›› 2011, Vol. 27 ›› Issue (10) : 968-971.
Research Papers

Overexpression of Fucosyltransferase Ⅳ Promotes Embryonic Cells to Adhere to Uterine Cells

  • LIU Shuai, FAN Li-Xue, YANG Xue-Song, YAN Qiu*
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Abstract

The adhesion of embryonic cells to the uterine epithelial cells is critical for the successful implantation, but the role of fucosyltransferase Ⅳ(FUT4) on embryo adhesion to uterine epithelial cells remains unknown. Here, we utilized an in vivo implantation model which was composed of JAR and HEC-1A cells, to study the effect of FUT4 on the adhesion of JAR to HEC-1A cells. By FUT4 expression plasmid transfection, FUT4 mRNA and protein level was increased, and also LeY synthesis increased on HEC-1A cell surface, which enhanced the percent adhesion of JAR to HEC-1A cells. These finding suggested that upregulation of FUT4 increased the synthesis of LeY on HEC-1A cell surface, and enhanced the adhesion of JAR to HEC-1A cells.

Key words

fucosyltransferase Ⅳ(FUT4) /  epithelium / embryo / Adhesion

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LIU Shuai, FAN Li-Xue, YANG Xue-Song, YAN Qiu. Overexpression of Fucosyltransferase Ⅳ Promotes Embryonic Cells to Adhere to Uterine Cells[J]. Chinese Journal of Biochemistry and Molecular Biology, 2011, 27(10): 968-971

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Funding

Supported by National Natural Science Foundation of China (No.31070729)

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