［1］   Williamson D J, Banik-Maiti S, DeGregori J, et al. hnRNP C is required for postimplantation mouse development but is dispensable for cell viability［J］. Mol Cell Biol, 2000, 20(11): 4094-4105 ［2］   Lynch K W. Consequences of regulated pre-mRNA splicing in the immune system［J］. Nat Rev Immunol, 2004, 4(12): 931-940 ［3］   Venables J P. Aberrant and alternative splicing in cancer［J］. Cancer Res, 2004, 64(21): 7647-7654 ［4］   Schwerk C, Schulze-Osthoff K. Regulation of apoptosis by alternative pre-mRNA splicing［J］. Mol Cell, 2005, 19(1): 1-13 ［5］   Srebrow A, Kornblihtt A R. The connection between splicing and cancer［J］. J Cell Sci, 2006, 119(Pt 13): 2635-2641 ［6］   Mry T, Heyd F. The impact of alternative splicing in vivo: Mouse models show the way［J］. RNA, 2007, 13(8): 1155-1171 ［7］   Caceres J F, Krainer A R, Kornblihtt A R. The pathology of pre-mRNA splicing: a meeting in the Italian Alps. Workshop on pre-mRNA processing and disease［J］. EMBO Rep, 2007, 8(8): 730-734 ［8］   Dassah M A, Patzek S, Hunt V M, et al. A genetic screen for suppressors of a mutated 59 splice site identifies factors associated with later steps of spliceosome assembly［J］. Genetics, 2009, 182(3): 725- 734 ［9］   Ohi M D, Link A J, Ren L, et al. Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs［J］. Mol Cell Biol, 2002, 22(7): 2011-2024 ［10］   Dix I, Russell C S, O’Keefe R T, et al. Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae［J］. RNA, 1998, 4(12): 1675-1686 ［11］   Dix I, Russell C, Yehuda S, et al. The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae［J］. RNA, 1999, 5(3): 360-368 ［12］   Vidal V P, Verdone L, Mayes A E, et al. Characterization of U6 snRNA-protein interactions［J］. RNA, 1999, 5(11): 1470-1481 ［13］   Duan J Z, Zhang J P, Zhu S X. mED2—a novel gene involved in mouse embryonic development［J］. Acta Genet Sin, 2006, 33(8): 692-701 ［14］   Nagy A, Gertsenstei M, Vintersten K, et al. Manipulating the Mouse Embryo: A Laboratory Manual ［M］. 3rd ed. New York: Cold Spring Harbor Laboratory Press, 2004 ［15］   NIH image software, Version1.32J, NIH, Bethesda, MD, USA ［16］   Flach G, Johnson M H, Braude P R, et al. The transition from maternal to embryonic control in the 2-cell mouse embryo［J］. EMBO J, 1982, 1(6): 681-686 ［17］   Bolton V N, Oades P J, Johnson M H. The relationship between cleavage, DNA replication, and gene expression in the mouse 2-cell embryo［J］. J Embryol Exp Morphol, 1984, 79: 139-163 ［18］   Black D L. Mechanisms of alternative pre-messenger RNA splicing［J］. Annu Rev Biochem, 2003, 72: 291-336 ［19］   Matlin A J, Clark F, Smith C W. Understanding alternative splicing: towards a cellular code［J］. Nat Rev Mol Cell Biol, 2005, 6(5): 386-398 ［20］   Grosso A R, Gomes A Q, Barbosa-Morais N L, et al. Tissue-specific splicing factor gene expression signatures［J］. Nucleic Acids Res, 2008, 36(15): 4823-4832 ［21］   Wang Z, Burge C B. Splicing regulation: From a parts list of regulatory elements to an integrated splicing code［J］. RNA, 2008, 14(5): 802-813 ［22］   Rio D C. Splicing of pre-mRNA: mechanism, regulation and role in development［J］. Curr Opin Genet Dev, 1993, 3(4): 574-584 ［23］   邵红伟, 张文峰, 胡青莲, 等. 两种存活蛋白变异剪接体在HeLa细胞中的表达定位及相互作用［J］.中国生物化学与分子生物学报(Shao Hong-Wei, Zhang Wen-Feng, Hu Qing-Lian,et al. Expression and location of two survivin splice variants and their interaction in HeLa cells［J］. Chin J Biochem Mol Biol), 2009, 25(12):1131-1136 ［24］   王兴平,罗仍卓么,许尚忠,等.牛IRAK2 基因有两种选择性剪接表达产物［J］. 中国生物化学与分子生物学报(Wang Xing-Ping, Luoren Zhuo-Ma, Xu Shang-Zhong, et al. Bovine IRAK2 gene encodes two alternatively spliced isoforms［J］. Chin J Biochem Mol Biol), 2009,25(3):292-296 ［25］   Fic W, Juge F, Soret J, et al. Eye development under the control of SRp55/B52-mediated alternative splicing of eyeless［J］. PLoS ONE, 2007, 2(2): e253 ［26］   Mordes D, Luo X, Kar A, et al. Pre-mRNA splicing and retinitis pigmentosa［J］. Mol Vis, 2006, 12: 1259-1271 ［27］   Fujita M, Kawano T, Terashima K, et al. Expression of spliceosome-associated protein 49 is required for early embryogenesis in Caenorhabditis elegans［J］. Biochem Biophys Res Commun, 1998, 253 (1): 80-84 ［28］   Ruiz-Lozano P, Doevendans P, Brown A, et al. Developmental expression of the murine spliceosome- associated protein mSAP49［J］. Dev Dyn, 1997, 208(4): 482-490