Construction and Evaluation of Lentiviral Vector pITA-HBP1

CHEN Yue-Ping, DU Yi-Cong, DONG Wei, WANG Wei-Bin, ZHANG Xiao-Wei

Chinese Journal of Biochemistry and Molecular Biology ›› 2010, Vol. 26 ›› Issue (11) : 1044-1049.

PDF(443 KB)
PDF(443 KB)
Chinese Journal of Biochemistry and Molecular Biology ›› 2010, Vol. 26 ›› Issue (11) : 1044-1049.
Research Papers

Construction and Evaluation of Lentiviral Vector pITA-HBP1

  • CHEN Yue-Ping1), DU Yi-Cong2), DONG Wei3),WANG Wei-Bin4), ZHANG Xiao-Wei4)*
Author information +
History +

Abstract

Lentiviral vectors have been widely used in gene therapy and in laboratory biological study for their ability to deliver larger gene fragments into cells in the non-dividing phase. In this study, we constructed a lentiviral vector pITA-HBP1 expressing the HBP1 transcription factor and evaluated its infection efficiency of the packaged lentivirus by Western blot. We found that both human tumor cells and normal cells were infected efficiently as determined by the expression of HBP1. Our results showed that HBP1 could be transduced exogenously into normal or cancer cells with our lentiviral system.

Key words

lentiviral vector
/ transcription factor HBP1 / infection efficiency
 

Cite this article

Download Citations
CHEN Yue-Ping, DU Yi-Cong, DONG Wei, WANG Wei-Bin, ZHANG Xiao-Wei. Construction and Evaluation of Lentiviral Vector pITA-HBP1[J]. Chinese Journal of Biochemistry and Molecular Biology, 2010, 26(11): 1044-1049

References

[1]   Tang H, Kuhen KL, Flossie WS. Lentivirus replication and regulation[J]. Annu Rev Genet, 1999, 33: 133-170 [2]   Asparuhova M B, Barde I, Trono D, et al. Development and characterization of a triple combination gene therapy vector inhibiting HIV-1 multiplication[J]. J Gene Med, 2008, 10(10):1059-1070 [3]   Shizuko S. Peptide nucleic acids as epigenetic inhibitors of HIV-1[J]. Int J Peptide Res Ther, 2005, 10(3):269-286 [4]   Sung MH, Simon R. Genomewide conserved epitope profiles of HIV-1 predicted by biophysical properties of MHC binding peptides [J]. J Comput Biol, 2004, 11(1):125-145 [5]   Cockrell AS, Kafri T. Gene delivery by lentivirus vectors[J]. Mol Biotechnol, 2007, 36(3): 184-204 [6]   Kock N, Kasmieh R, Weissleder R, et al. Tumor therapy mediated by lentiviral expression of shBcl-2 and S-TRAIL[J]. Neoplasia, 2007, 9(5):435-442 [7]   Delenda C. Lentiviral vectors: optimization of packaging transduction and gene expression[J]. Gene Med, 2004,6(1): 125-138 [8]   Rossi GR, Mautino MR, Morgan RA. High-efficiency lentiviral vector-mediated gene transfer into murine macrophages and activated splenic B lymphocytes[J]. Hum Gene Ther, 2003,14(4): 385-391 [9]   Moffat J, Grueneberg D A, Yang X, et al. A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen[J]. Cell, 2006, 124(6): 1283-1298 [10]   Heldt SA, Ressler KJ. Amygdala-specific reduction of alpha1-GABAA receptors disrupts the anticonvulsant, locomotor, and sedative, but not anxiolytic, effects of benzodiazepines in mice [J]. J Neurosci, 2010, 30(21): 7139-7151 [11]   Xu CY, Gu Y, Hou WH,et al. Tetracysteine as a reporter for gene therapy[J]. Biomed Environ Sci, 2009, 22(6): 496-501 [12]   Zhang X, Kim J, Ruthazer R, et al. The HBP1 transcriptional repressor participates in Ras- induced premature senescence[J]. Mol Cell Biol, 2006,26(22):8252-8266 [13]   Wu Y, Beddall MH, Marsh JW. Rev-dependent lentiviral expression vector[J]. Retrovirology, 2007, 4:12-16 [14]   Dull T, Zufferey R, Kelly M, et al. A third-generation lentivirus vector with a conditional packaging system[J]. J Virol, 1998, 72(11):8463-8471 [15]   Mautino MR. Lentiviral vectors for gene therapy of HIV-1 infection[J]. Curr Gene Ther, 2002, 2(1):23-43

Funding

Supported by General Program from National Natural Science Foundation of China (No. 30770442)
PDF(443 KB)

389

Accesses

0

Citation

Detail

Sections
Recommended

/