Mitogen-inducible gene 6 (MIG-6) induces normal human fibroblast cell premature

ZHAO Lin, ZHUO De-Xiang, MAO Ze-Bin-*

Chinese Journal of Biochemistry and Molecular Biology ›› 2010, Vol. 26 ›› Issue (10) : 918-922.

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Chinese Journal of Biochemistry and Molecular Biology ›› 2010, Vol. 26 ›› Issue (10) : 918-922.
Research Papers

Mitogen-inducible gene 6 (MIG-6) induces normal human fibroblast cell premature

  • ZHAO Lin, ZHUO De-Xiang, MAO Ze-Bin-*
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Abstract

In senescent cells, gene expression is different from that in young cells and some of them can induce human fibroblast cell premature. Oncogene-induced senescence (OIS) is a robust and sustained anti-proliferative response brought about by oncogenic signaling. Evidence shows that mitogen-inducible gene 6 (MIG-6) is a tumor suppressor gene. MIG-6 acts as a negative feedback regulator of the ErbB RTK pathway, and inhibits tumor cell proliferation. In this article we used human embryonic lung diploid fibroblast cell as a model to study the role of MIG-6 in the progress of cell senescence. Through Western blotting we found that the expression of MIG-6 in senescent cells increased. We constructed retroviral MIG-6 cDNA vector and transfected it into normal human fibroblast cells and analyzed MIG-6 expression in transfected cells. Then we examined the β-gal activity of HDF cells transfected with MIG-6 cDNA and found that the ratio of stained cells in transfected cells was much more than control cells, and the growth rate decreased. It was concluded that the over-expression of MIG-6 protein induced human embryonic lung diploid fibroblast cell premature.

Key words

mitogen-inducible gene-6(MIG-6) / cell senescence / human embryonic lung diploid fibroblasts

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ZHAO Lin, ZHUO De-Xiang, MAO Ze-Bin-*. Mitogen-inducible gene 6 (MIG-6) induces normal human fibroblast cell premature[J]. Chinese Journal of Biochemistry and Molecular Biology, 2010, 26(10): 918-922

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Funding

Supported by Major State Basic Research Development Program of China (973 Program, No. 2007CB507400)

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