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Novel Preadipocyte Cell Model Established via Pig Mature Adipocytes
LIHui-Xia,;LUOXiao;LIURong-Xin;YANGGong-She
Chinese Journal of Biochemistry and Molecular Biology ›› 2009, Vol. 25 ›› Issue (03) : 270-275.
PDF(471 KB)
PDF(471 KB)
Novel Preadipocyte Cell Model Established via Pig Mature Adipocytes
LI Hui-Xia1), 2 ), LUO Xiao1), LIU Rong-Xin1),YANG Gong-She 1)*
The adipose tissue isolated from the 1～3 day piglets subcutaneouly, and mature adipocytes were cultured by “ceiling culture" method. Morphological changes in different culture days were observed, cell proliferation was measured by cell count method, and the cycles were analyzed by flow cytometry (FCM). Via oil red O staining assess the degree of adipogenesis and differentiation was assessed, the gene expression of preadipocyte factor-1(Pref-1), peroxisome proliferatoractivated receptor-γ (PPARγ) and CCAAT/enhancer binding protein-α (C/EBPα) was detected by reverse transcriptasepolymerase chain reaction (RT-PCR). The results showed that the primal adherences cells were mature adipocytes with imple lipids, and completed positive with oil red O staining, the mature adipocytes dedifferentiated to fiber cell without any lipids after 14 days. The fiber cells expressed preadipocytes marker Pref-1 but not myogenic regulatory protein (MyoD), and negative with oil red O staining. The de-differentiated fiber cell can redifferentiated into mature adipocytes with lipids, and express adipocytes markers PPAR-γ and C/EBPα when cultured in adipogenesis inducer mediums. The results indicate that the dedifferentiated preadipocytes can redifferentiated into mature adipocytes with ability of proliferation, and it may be a valid preadipocyte cell model.
mature adipocytes / ceiling culture / proliferation / differentiation / preadipocyte {{custom_keyword}} /
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