
Effect of Recombinant Buckwheat Trypsin Inhibitor on Apoptosis and
Caspases Activity in Human Hepatoma (HepG2) Cells
LIFang;LIYu-Ying;BAIChong-Zhi;TIANXin;ZHANGZheng;WANGZhuan-Hua
Chinese Journal of Biochemistry and Molecular Biology ›› 2009, Vol. 25 ›› Issue (02) : 182-187.
Effect of Recombinant Buckwheat Trypsin Inhibitor on Apoptosis and
Caspases Activity in Human Hepatoma (HepG2) Cells
LI Fang;LI Yu-Ying;BAI Chong-Zhi;TIAN Xin;ZHANG Zheng;WANG Zhuan-Hua*
Recombinant buckwheat trypsin inhibitor (rBTI) has been reported to induce apoptosis in human cancer cells,such as IM-9,K562.To examine whether rBTI had an anticancer effect on HepG2 human hepatoma cells,and reveal the possible mechanisms,cells treated with rBTI were subjected to MTT,nuclear staining and DNA fragmentation assays for measuring growth inhibition and induction of apoptosis.In addition,as the Bcl-2 (an anti-apoptotic gene)/Bax (a pro-apoptotic gene) ratio is associated with the increase of apoptosis,the mRNA expression levels of both were determined.Following rBTI incubation,Bcl-2 was decreased,whereas Bax was increased.We also investigated the alteration of the Fas/FasL system after rBTI treatment in HepG2 cells. However,not significant changes were found.rBTI treatments markedly induced the activities of caspase-3 and caspase-9,while caspase-8 was responded slightly.These findings suggested that rBTIinduced apoptosis was likely initiated at mitochondria and maybe related to the caspase-3 signaling pathway,but not via Fas/FasL.
recombinant buckwheat trypsin inhibitor / apoptosis / HepG2 cell / caspases {{custom_keyword}} /
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