E2F1 Mediates 8-Cl-Adenosine-induced Apoptosis in Human Lung Cancer Cells H1299

FENGLi-Yan,YANGSheng-Yong,ANGuo-Shun,LIShu-Yan,NIJu-Hua,JIAHong-Ti,

Chinese Journal of Biochemistry and Molecular Biology ›› 2008, Vol. 24 ›› Issue (09) : 861-865.

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Chinese Journal of Biochemistry and Molecular Biology ›› 2008, Vol. 24 ›› Issue (09) : 861-865.
Research Papers

E2F1 Mediates 8-Cl-Adenosine-induced Apoptosis in Human Lung Cancer Cells H1299

  • FENG Li-Yan1)**, YANG Sheng-Yong2)**, AN Guo-Shun3), LI Shu-Yan3), NI Ju-Hua3), JIA Hong-Ti1),3)*
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Abstract

In this article, we intend to investigate the mechanism of 8-Cl-adenosine (8-Cl-Ado)induced apoptosis in human non-small cell lung carcinoma H1299 cells. MTT assay was first employed to determine the anti-proliferation effects of 8-Cl-Ado; Western blotting was used to examine the activation of procaspase-3 and the levels of E2F1 after 8-Cl-Ado exposures. After transfecting pcDNA-HA-E2F1 (E2F1 overexpression) or pSUPER-E2F1 (RNAi of E2F1) vector into H1299 cells, the procaspase-3, as a marker of apoptotic cell death, as well as E2F1 was determined by Western blotting. The results showed that 8-Cl-Ado induced growth inhibition in H1299 cells, by the activation of procaspase-3 and the up-regulation of E2F1. Procaspase-3 was activated in response to E2F1 over-expression, and markedly inhibited following E2F1 knocke-down by RNAi. Our data suggested that E2F1 mediated 8-Cl-Adoinduced apoptosis in human lung cancer cells H1299.

Key words

8-Cl-adenosine / E2F1 / apoptosis / non-small cell lung carcinoma

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FENGLi-Yan,YANGSheng-Yong,ANGuo-Shun,LIShu-Yan,NIJu-Hua,JIAHong-Ti,. E2F1 Mediates 8-Cl-Adenosine-induced Apoptosis in Human Lung Cancer Cells H1299[J]. Chinese Journal of Biochemistry and Molecular Biology, 2008, 24(09): 861-865

References

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