Proline-rich Region in Immune Suppression Protein SOCS3 is Required for Its Biological Functions

LIHai-Jie,ZENGBin,ZHANGZhuo-Han,WANGLi-Na,ZHANGZhu-Hong,YANGRong-Cun

Chinese Journal of Biochemistry and Molecular Biology ›› 2008, Vol. 24 ›› Issue (05) : 432-438.

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Chinese Journal of Biochemistry and Molecular Biology ›› 2008, Vol. 24 ›› Issue (05) : 432-438.
Research Papers

Proline-rich Region in Immune Suppression Protein SOCS3 is Required for Its Biological Functions

  • LI Hai-Jie,ZENG Bin,ZHANG Zhuo-Han,WANG Li-Na,ZHANG Zhu-Hong, YANG Rong-Cun*
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Abstract

Suppressor of cytokine signaling 3 (SOCS3) negatively regulates signal transduction and exerts an important role in regulating the differentiation of hematopoietic cells. SOCS3 contains an praline-rich region as determined by bioinformatics-based analysis.To determine the role of this praline-rich region, the deletion mutant of SOCS3 amino acids 129~161 (SOCS3Δ129-161) was obtainde using PCR and then cloned into pcDNA31/V5 recombinant plasmid.The functions of SOCS3Δ129-161were analyzed by flow cytometry in transfected cells. The results showed that the threedimensional structure of SOCS3Δ129-161 was clearly different from that of wildtype as predicted by comparative(homology) computation modeling.In addition, the SOCS3Δ129-161 abrogated the functions of native SOCS3, inducing CD8+T cell differentiation. The results suggested that the praline-rich region within amino acids 129~161 of SOCS3 might play an important role in the formation and maintaining of SOCS3 tertiary structure and significantly contributed for its biological functions.

Key words

SOCS3 / tertiary structure modeling / recombinant PCR / FACS

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LIHai-Jie,ZENGBin,ZHANGZhuo-Han,WANGLi-Na,ZHANGZhu-Hong,YANGRong-Cun. Proline-rich Region in Immune Suppression Protein SOCS3 is Required for Its Biological Functions[J]. Chinese Journal of Biochemistry and Molecular Biology, 2008, 24(05): 432-438

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