Quantitative Analysis of Mitochondrial Hydrophobic Proteomein HCPT-Induced Apoptosis of Hepatoma Cells

YANYu-Rong;FUYu-Rong;WANGWei-Jia,;QIUZong-Yin

Chinese Journal of Biochemistry and Molecular Biology ›› 2008, Vol. 24 ›› Issue (04) : 373-382.

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Chinese Journal of Biochemistry and Molecular Biology ›› 2008, Vol. 24 ›› Issue (04) : 373-382.
Research Papers

Quantitative Analysis of Mitochondrial Hydrophobic Proteomein HCPT-Induced Apoptosis of Hepatoma Cells

  • YAN Yu-Rong;FU Yu-Rong; WANG Wei-Jia,;QIU Zong-Yin*

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Abstract

Hydroxycamptothecin is an anticancer agent that is known to induce apoptosis in hepatoma cells through the mitochondrial pathway. To extend the study of the molecular mechanism involved in hydroxycamptothecininduced-apoptosis, we analyzed the differentially expressed mitochondrial hydrophobic proteins in hydroxycamptothecin-treated cells via a quantitative proteomics approach. The mitochondria extracts from hydroxycamptothecin treated hepatoma cells were analyzed by western blot; and the mitochondrial hydrophobic proteins were labeled with cleavableisotopecoded affinity tag(c-ICAT)reagent. A total of 144 proteins were significantly (P<0.05) altered in hydroxycamptothecintreated cells; they were subsequently identified and quantified based on a shotgun proteomic method using a multiple dimensional liquid chromatography-linear ion trap/orbitrap mass spectrometer. 12 proteins were down-regulated after hydroxycamptothecin treatment, whereas 43 proteins were up-regulated for more than 10-folds. The functions of these proteins include cell proliferation, differentiation, apoptosis, energy metabolism, nucleic acid synthesis and signal transductions. The results may help further investigation of the pharmacological impacts in hydroxycamptothecin treated cells.

Key words

mitochondrion / hydrophobic protein / c-ICAT / hydroxycamptothecin / subcellular quantitative proteomics

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YANYu-Rong;FUYu-Rong;WANGWei-Jia,;QIUZong-Yin. Quantitative Analysis of Mitochondrial Hydrophobic Proteomein HCPT-Induced Apoptosis of Hepatoma Cells

[J]. Chinese Journal of Biochemistry and Molecular Biology, 2008, 24(04): 373-382

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