Differential Expression and Principal Component Analysis
of Genes Related to Muscle Growth and Adipose Deposition

LIMing-Zhou,LIXue-Wei,ZHULi,TENGXiao-Kun,XIAOHua-Sheng,LIQiang,CHENLei

Chinese Journal of Biochemistry and Molecular Biology ›› 2008, Vol. 24 ›› Issue (01) : 46-54.

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PDF(446 KB)
Chinese Journal of Biochemistry and Molecular Biology ›› 2008, Vol. 24 ›› Issue (01) : 46-54.
Research Papers

Differential Expression and Principal Component Analysis
of Genes Related to Muscle Growth and Adipose Deposition

  • LI Ming-Zhou 1), LI Xue-Wei 1)*, ZHU Li 1), TENG Xiao-Kun 2), XIAO Hua-Sheng2), LI Qiang 1), CHEN Lei 1)
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Abstract

The competitive equilibrium of growth rate of skeletal muscle cells and adipose cells determines porcine meat quality and carcass traits simultaneously. The expression changes of genes associated with muscle growth and adipose deposition in longissimus dorsi muscle at six growth stages (birth, 1, 2, 3, 4 and 5 months) of Landrace and Taihu pigs were examined by using pathway-focused Oligo microarray. The comparative results of change ratios between two pig breeds showed that the fold changes of 15, 16, 11, 13, 18 and 20 genes were over two times at same month from birth to 5 month, respectively. Variance analysis (ANOVA) revealed that differences in the expression of 18 genes in Landrace pigs and 3 genes in Taihu pigs were very significant (P<0.01) and differences for 22 genes in Landrace pigs and 7 genes in Taihu pigs were significant (P<0.05) among six growth stages. Principal component analysis (PCA) revealed that the most representative gene expression pattern of both two pig breeds represented genes which were first down-regulated strongly, and then up-regulated gradually. In addition, 7 genes of Landrace and 6 genes of Taihu pigs displayed distinctive expression pattern from other genes, which suggested that these genes maybe came under special regulation during the growth and development of skeletal muscle cells and adipose cells. Quantitative real-time RT-PCR was used to verify the microarray data for five modulated genes, and a good positive correlation between the two measures of expression was observed for both two pig breeds at different growth stages (r=0.876±0.095).These results highlight some possible candidate genes for porcine meat quality and carcass traits and provide some data on which to base further study of the expressional disciplinarian and molecular mechanism of genes related to muscle fiber growth and fatty acid biosynthesis.

Key words

pig / microarray / muscle / adipose / gene differential expression / principal component analysis

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LIMing-Zhou,LIXue-Wei,ZHULi,TENGXiao-Kun,XIAOHua-Sheng,LIQiang,CHENLei. Differential Expression and Principal Component Analysis
of Genes Related to Muscle Growth and Adipose Deposition
[J]. Chinese Journal of Biochemistry and Molecular Biology, 2008, 24(01): 46-54

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