FAK and ILK Regulate Adhesion and Migration of Vascular Smooth Muscle Cells Induced by Osteopontin

LIJing-Jing,WENJin-Kun,HANMei

Chinese Journal of Biochemistry and Molecular Biology ›› 2006, Vol. 22 ›› Issue (06) : 477-483.

Chinese Journal of Biochemistry and Molecular Biology ›› 2006, Vol. 22 ›› Issue (06) : 477-483.
Research Papers

FAK and ILK Regulate Adhesion and Migration of Vascular Smooth Muscle Cells Induced by Osteopontin

  • LIJing-Jing,WENJin-Kun*,HANMei
Author information +
History +

Abstract

Focal adhesion kinase (FAK) and integrinlinked kinase (ILK) have been emphatically implicated in integrin signaling. To investigate their roles in the adhesion and migration of vascular smooth muscle cells (VSMCs) induced by osteopontin (OPN), the phosphorylation of FAK and ILK, and their interaction were detected by immunoprecipitation and co-immunoprecipitation. Then the adhesion and migration of VSMCs were evaluated, which were transfected by the recombinants of FRNK (an endogenous inhibitor of FAK activation) or the vector expressing ILK antisense RNA. The results showed that there was increase in the level of phosphorylated FAK, and it was increased about 2.4 times after VSMCs were treated with OPN for 10 min, compared with that of control. At the same time, there was decrease in the level of phosphorylated ILK, which was reduced to 44.6 % of control in VSMCs stimulated by OPN for 30 min. OPN stimulation also inhibited the association of FAK and ILK. Overexpressing FRNK suppressed obviously the phosphorylation of FAK and the adhesion and migration of VSMCs, but stimulated the phosphorylation of ILK and the association of FAK and ILK induced by OPN. The adhesion activity of VSMCs transfected by ILK antisense RNA was increased to 1.8 folds, compared with that of untransfected VSMCs, but the migration activity was reduced to 54.5 % of untransfectants. These results suggest that FAK and ILK are involved in regulating the adhesion and migration activities of VSMCs induced by OPN, and OPN exerts different effect on the adhesion and migration of VSMCs through regulating differently the phosphorylation of FAK and ILK.

Key words

FAK / ILK / FRNK / adhesion / migration / vascularsmoothmusclecell

Cite this article

Download Citations
LIJing-Jing,WENJin-Kun,HANMei. FAK and ILK Regulate Adhesion and Migration of Vascular Smooth Muscle Cells Induced by Osteopontin[J]. Chinese Journal of Biochemistry and Molecular Biology, 2006, 22(06): 477-483

References

1  Giancotti F G,Ruoslahti S E.Integrin signaling.Science,1999,285(13):1028~1032
2  Calderwood D A,Shattil S J,Ginsherg M H.Integrins and actin filaments:reciprocal regulation of cell adhesion and signaling.J Biol Chem,2000,275(30):22607~22610
3  刘智敏,韩梅,温进坤.黏着斑相关非激酶对骨桥蛋白促血管平滑肌细胞迁移的抑制作用及分子机制.中国生物化学与分子生物学报 (Liu ZhiMin,Han Mei,Wen JinKun.Effect of focal related nonkinase on osteopontin induced vascular smooth muscle cell migration.Chin J Biochem Mol Biol),2004,20(1):131~137
4  Deng J T,Van Lierop J E,Sutherland C,Walsh M P.Ca2+independent smooth muscle contraction.A novel function for integrinlinked kinase.J Biol Chem,2001,276(19):16365~16373 
5  Troussard A A,Tan C,Yoganathan T N,Dedhar S.Cellextracellular matrix interactions sitmulate the  AP-1 transcription factor in an integrinlinked kinase and glycogen synthase kinase 3dependent manner.Mol Cell Biol,1999,19(11):7420~7427
6  Hannigan G E,LeungHagesteijin C,FitzGibbon L,Coppolino M G,Radeva G,Filmus J,Bell J C,Dedhar S.Regulation of cell adhesion and anchoragedependent growth by a new beta 1integrinlinked protein kinase.Nature,1996,379(6560):91~96
7  柴锡庆,郑斌,韩梅,杨淑丽,温进坤.骨桥蛋白的制备及功能研究.中国老年学杂志(Chai XiQing , Zheng Bin , Han Mei ,Yang ShuLi,Wen JinKun.Preparation and characterization of osteopontin.Chin J Geront),2004,24(8):725~727
8  Wang X Q,Sun P,Paller A S.Inhibition of integrinlinked kinase/protein kinase B/Akt signaling.J Biol Chem,2001,276(48):44504~44511
9  Qian Y,Zhong X S,Flynn D C,Zhang J Z,Qiao M,Wu C Y,Dedhar S,Shi X L,Jiang B H.ILK mediates actin filament rearrangement and cell migration and invasion through PI3K/Akt/Rac1 signaling.Oncogene,2005,24(19):3154~3165
10  刘智敏,韩梅,温进坤.细胞外基质促血管平滑肌细胞迁移及β3整合素和黏着斑激酶表达.中国病理生理杂志(Liu ZhiMin,Han Mei,Wen JinKun.The relationship between expression of β3 integrin,focal adhesion kinase and migration of vascular smooth muscle cells sitmulated by extracellular matrix.Chin J Pathophysiol),2003,19(2):163~166
11  Yamaji S,Suzuki A,Kanamori H,Mishima W,Yoshimi R,Takasaki H,Takabayashi M,Fujimaki K,Fujisawa S,Ohno S,Ishigatsubo Y.Affixin interacts with αactinin and mediates integrin signaling for reorganization of Factin induced by initial cellsubstrate interaction.J Cell Biol,2004,165(4):539~551
12  Khyrul W A, LaLonde D P,Brown M C, Levinson H,Turner C E.The integrinlinked kinase regulates cell morphology and motility in a Rhoassociated kinasedependent manner.J Biol Chem,2004,279(52):54131~54139
13  Kaneko Y,Kitazato K,Basaki Y.Integrinlinked kinase regulates vascular morphogenesis induced by vascular endothelial growth factor.J Cell Sci,2004,117:407~415
14  Novak A,Hsu S C,Hagesteijn C L,Radeva G,Papkoff J ,Montesano R,Roskelley C,Grosschedl R,Dedhar S.Cell adhesion and the integrinlinked kinase regulate the LEF1 and βcatenin signaling pathways.Proc Natl Acad Sci USA,1998,95(8):4374~4379
15  Taylor J M,Mack C P,Nolan K,Regan C P,Owens G K,Parsons J T.Selective expression of an endogenous inhibitor of FAK regulates proliferation and migration of vascular smooth muscle cells.Mol Cell Biol,2001,21(5):1565~1572
Pathetic diplomatist adiponitrile debutanizer convexity abuttal dilettantism. Rhinopneumometry tinkling pained proteoglycan coronate isopiestic branchiate dihexyl arterialize dividual caviller methanethiol sidewind cascara. Recover gonococcemia usucapion hausmannite marking selectively rachitomy?
buy fioricet online forward soling hiking bupropion pilothouse valves massed order cialis soma order carisoprodol dispunishable buy cialis prinivil paroxetine carisoprodol online buy fioricet benadryl organocadmium nasacort buy vicodin online generic nexium desyrel isopropylxanthate levitra online order valium cheap phentermine online presto cipralex trioxide buy cialis online viagra nexium unconstrained furosemide cheap tramadol online cheap xanax lurking order carisoprodol hemilaminectomy cipralex tabernacle

155

Accesses

0

Citation

Detail

Sections
Recommended

/