Differential Proteomic Analysis of Liver of Treble Lipid Metabolism Genes Mutant Mouse

FUQiang;GUDaiJun;SHENLongXiang;DUWenXi;WANGNa;SONGXingHui; JINXiaoLei;PAN Jie 

Chinese Journal of Biochemistry and Molecular Biology ›› 2006, Vol. 22 ›› Issue (05) : 396-401.

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Chinese Journal of Biochemistry and Molecular Biology ›› 2006, Vol. 22 ›› Issue (05) : 396-401.
Research Papers

Differential Proteomic Analysis of Liver of Treble Lipid Metabolism Genes Mutant Mouse

  • FUQiang1)**;GUDaiJun1)**; SHENLongXiang3);DUWenXi3);WANGNa1);SONGXingHui1); JINXiaoLei1) ;PAN Jie 2)
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Abstract

Two-dimensional gel electrophoresis and mass spectrometry have been used for analysis differential displayed proteomics of 5 week-old treble lipid metabolism genes mutant mice (apoE-1-/LDLR-1-/Leprdb/db) and wild type mice.Comparative study of differential expression of protein profiles of livers between treble lipid metabolism genes mutant mice and wild type mice. The key protein related to atherosclerosis and dysfunction of lipid metabolism also characterized.Approximately (841±57) spots and (1 017±50) spots have been detected in treble lipid metabolism genes mutant mice livers (n=3) and wild type mice livers (n=3). The average matched ratio was 71.9% and 83.2%. The differential expression analysis showed that there were matched spots between treble lipid metabolism genes mutant mice and wild type mice. Compared with wild type, 140 spots were not matched in treble lipid metabolism genes mutant mice. 7 over expression spots (>5 fold) and 39 lower expression spots (>5 fold) were noted. Six significant differentially proteins in gel were identified by LTQ_ESI, e.g.endoplasmin precursor (Grp94),acidic leucin_rich nuclear phosphoprotein 32 family member A, serotransferrin precursor, fructose bisphosphatase 1, fibronectin precursor, complement C3 precursor, fibrinogen B beta polypeptide.The protein profile of treble lipid metabolism genes mutant mice livers exhibited significant difference compared to that of wild type mice. The results implied that lipid metabolism relative polygenetic mutation contribute to the alteration of mouse liver protein expression profile,especially which lipid metabolism related and others participate in dysfunction in lipid metabolism during atherogenesis.

Key words

lipid metabolism genes: atherosclerosis: liver: two-dimensional electrophoresis: mass spectrometry analysis

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FUQiang;GUDaiJun;SHENLongXiang;DUWenXi;WANGNa;SONGXingHui; JINXiaoLei;PAN Jie . Differential Proteomic Analysis of Liver of Treble Lipid Metabolism Genes Mutant Mouse[J]. Chinese Journal of Biochemistry and Molecular Biology, 2006, 22(05): 396-401

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