Expression of ω-6 Fatty Acid Desaturase Has an Inhibition Effects on Breast Cancer Cells in vitro

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Chinese Journal of Biochemistry and Molecular Biology ›› 2005, Vol. 21 ›› Issue (03) : 329-333.

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Expression of ω-6 Fatty Acid Desaturase Has an Inhibition Effects on Breast Cancer Cells in vitro

GE Yin-Lin*, GENG Fang-Song, ZHANG Jin-Yu, ZHANG Zheng, LI Xin, TIAN Run-Hua, XU Wen-Hua

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To explore transformation of fat-1 gene encoding an ω-6 fatty acid desaturase into human breast cancer cell to inhibit the proliferation of the cells,the fat-1 cDNA was cloned into pAd-CMV vector to construct a recombinant adenovirus and transfected 293 cells to obtain Ad·GFP·fat-1 recombinant virus.Immunofluorescence microscopy found that positive green signal was appeared in 60%～70% of total cells after two days of infection of human breast cancer cell line MCF-7 with the recombinant virus,which was further confirmed at mRNA level by ribonuclease protection assay.At the same time,the proliferation of MCF-7 cells was significantly attenuated to 80% of control group as assessed by MTT assay.The underlying mechanism for reduction of cell growth may due to increase of apoptotic rate induced by the fat-1 gene expression:Hoechst dye staining following by showed that expression of fat-1 gene in maligment cell enhances the occurring of cell apoptosis up to 35%～50%.Measurement of prostaglandin E2 found that the concentration of the PGE2 also markedly reduced.These data provide a foundation for applying ω-6 fatty acid desaturase gene as gene therapy of human breast cancer.

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gene therapy / unsaturated fatty acids / human breast cancer cell

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GE Yin-Lin*, GENG Fang-Song, ZHANG Jin-Yu, ZHANG Zheng, LI Xin, TIAN Run-Hua, XU Wen-Hua. Expression of ω-6 Fatty Acid Desaturase Has an Inhibition Effects on Breast Cancer Cells in vitro[J]. Chinese Journal of Biochemistry and Molecular Biology, 2005, 21(03): 329-333

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Received	Revised	Published
2005-06-20	2005-06-20	2005-06-20
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2005-06-20

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