Thrombopoietin (TPO) could enhance the T lymphocyte proliferation and lymphokine productions in mice. Using a gene transfer model, the effects of TPO on murine sarcoma growth in vivo were evaluated and the possible mechanisms were investigated. TPO transfer (100 μg/mouse) 2 weeks ahead to ( P <0 05), or simultaneously ( P >0 05) with S180 cells implantation resulted in less tumor weight grown in and longer survival time of the mice. When 1×10 6 cells were implanted, the average tumor weights were 1 756 g,3 637 g and 3 926 g, with TPO transfer,null pcDNA3 plasmid transfer and saline water injection, respectively. And when 1×10 6 cells implanted, the 2 weeks average tumor weights were 0 576 g, 5 61 g and 5 84 g, respectively. Even more, 4/10 of the mice with TPO transfer ahead survived without tumor development or with the tumor growth being totally restrained (with 1×10\+5 cells implanted). TPO transfer and sera from TPO transferred mice could not suppress on the tumor cells in vitro . Phenotype analysis demonstrated that surface antigen of the dominant group of tumor infiltration lymphocytes shifted from CD8 + to CD4 + and/or CD4 +CD8 + after TPO gene therapy. Conclusively, the present data suggested that TPO over expression exerted tumor suppression and helper subset T lymphocytes activation involved in this process.
Key words
thrombopoietin /
gene therapy /
tumor suppression /
T lymphocyte
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References
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