Characteristics and Biological Properties of Ribosome Inhibiting Protein(RIP)from Momordica charantia (bitter melon seeds)

MENG Yanfa,DU Yifeng *, ZHANG Xuemei

Chinese Journal of Biochemistry and Molecular Biology ›› 1999, Vol. 15 ›› Issue (06) : 920-923.

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PDF(349 KB)
Chinese Journal of Biochemistry and Molecular Biology ›› 1999, Vol. 15 ›› Issue (06) : 920-923.
Article

Characteristics and Biological Properties of Ribosome Inhibiting Protein(RIP)from Momordica charantia (bitter melon seeds)

  • MENG Yanfa,DU Yifeng *, ZHANG Xuemei
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Abstract

A RIP was isolated and purified from the Momordica charantia seeds by precipitation of 30%-65% saturation of (NH 4) 2SO 4 followed by affinity chromatography on Cibacron Blue F3GA Sephadex G 100 and Sephacryl S 100 molecular sieve chromatography The results of SDS PAGE,PAGE,IEF PAGE and PAS Schiff staining after SDS PAGE showed that RIP was a homogeneous single band or a glycoprotein band with p I of 8 9-9 0 The molecular weight of 30 000 was determined by SDS PAGE and that of 28 000 by molecular sieve chromatography on Sephadex G 150 The total neutral sugar content of purified RIP expressed as mannose was 1 53% The RIP obtained from Momordica charantia had a powerful inhibitory action upon protein synthesis in reticulocyte lysate and IC 50 was 5 3×10 -10 mol/L The antitumor activity and cytotoxicity on intact cells were studied too These findings suggest that RIP may be a useful agent in the treatment of some tumors and virus infection

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MENG Yanfa,DU Yifeng *, ZHANG Xuemei. Characteristics and Biological Properties of Ribosome Inhibiting Protein(RIP)from Momordica charantia (bitter melon seeds)[J]. Chinese Journal of Biochemistry and Molecular Biology, 1999, 15(06): 920-923

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