Selenocysteine Insertion Sequence Elements in Glutathione Peroxidase cDNAs from Seven Species
QU Xianghu,HUANG Kaixun,GAO Zhonghong,XU Huibi
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(Department of Chemistry,Huazhong University of Science and Technology,Wuhan 430074
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Received
Revised
Published
1999-04-20
1999-04-20
1999-04-20
Issue Date
1999-04-20
Abstract
The biological functions of Se are mediated through selenoproteins with Se as selenocysteine.It is known that specific insertion of selenocysteine into proteins is directed by the UGA codon.In eukaryotes,co transtational insertion of selenocysteine into selenoproteins necessitated the participation of the selenocysteine insertion sequence (SECIS),an element lying in the 3' untranslated region (UTR) of selenoprotein mRNAs.Based on computer folding studies,the putative SECIS elements found in the 3'UTR of the 15 glutathione peroxidase (GPx)cDNAs were compared,which contained three conserved features:AUGA A(G)AA GA.Predicted secondary structures for the 15 SECIS elements belong to the form Ⅰ group(the unparied AAA nucleotides lie in the apical loop)or the form Ⅱ group (the unparied AAA form a bulge in the 5'arm).The 3'UTR of cytoplasmic GPx,human plasma GPx and Schistosoma mansoni GPx contain the form Ⅰ SECIS,while the 3'UTR of phospholipid hydroperoxide GPx,plasma GPx(rat,mouse and bovine)and human giGPx contain the form Ⅱ SECIS.
QU Xianghu,HUANG Kaixun,GAO Zhonghong,XU Huibi.
Selenocysteine Insertion Sequence Elements in Glutathione Peroxidase cDNAs from Seven Species[J]. Chinese Journal of Biochemistry and Molecular Biology, 1999, 15(02): 274-278
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