Studies on the Purification and Characterization of Superoxide Dismutase from Tartary Buckwheat Leaves

Wang Zhuan-hua; Zhang Zheng

Chinese Journal of Biochemistry and Molecular Biology ›› 1994, Vol. 10 ›› Issue (05) : 539-543.

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PDF(873 KB)
Chinese Journal of Biochemistry and Molecular Biology ›› 1994, Vol. 10 ›› Issue (05) : 539-543.
Article

Studies on the Purification and Characterization of Superoxide Dismutase from Tartary Buckwheat Leaves

  • Wang Zhuan-hua; Zhang Zheng
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Abstract

Copper and zinc-containing superoxide dismutase with high specific activity was prepared from fresh tartary buckwheat leaves. The molecular weight of the purified enzyme is about 31kD, a maximum UV-absorption of the enzyme is found at 259nm, and the analysis of amino acid composition showed that the enzyme contains 308 amino acid residues.The purified enzyme showed three isozyme bands by PAGE.

Key words

Tartary buckwheat leaves / Superoxide dismutase / Purification / Characterization

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Wang Zhuan-hua; Zhang Zheng. Studies on the Purification and Characterization of Superoxide Dismutase from Tartary Buckwheat Leaves[J]. Chinese Journal of Biochemistry and Molecular Biology, 1994, 10(05): 539-543

References

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