Using DNA immunoassays we have shown that the 5'-flanking region of the genes encoding human Ul and U2 snRNA contain regions that are able to bind tightly and specifically with the SV40 large tumor antigen (T Ag). The affinity of T Ag to U2 gene is greater than that to Ul gene. DNase I footprinting showed that 5'-flanking regions of both Ul and U2 contain sequences that were specifically protected against digestion by T Ag. About 30nt regions on either strand of DNA in human Ul and U2 genes were protected by T Ag. These regions were located at -11 to -42 bp on Ul gene and -58 to -90 bp on U2 gene.
Key words
SV40 T antigen /
U1 /
U2 snRNA gene /
DNA immunoassay /
Dnase I footprinting
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References
[1] Fried M and Prives C. Cancer Cell 4/DNA Tumor Virases, Cold Spring Harbor Laboratory, New York, 1986, 1-16
[2] Delucia A L et al. J Virol, 1983, 46: 216-222
[3] Murphy J T et al. Cell, 1982, 29: 265-274
[4] Westin G et al. EMBO, 1984, 3: 3295-3301
[5] Prives C et al. Mol Cell Biol, 1983, 3: 1958-1966
[6] Hansen U et al. Cell, 1981, 27: 603-612
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Footnotes
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