ANALYSIS OF THE NUCLEOTlDE SEQUENCE OF CORE ANTIGEN GENE OF Adr SUBTYPE HEPATITIS B VIRUS

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Chinese Journal of Biochemistry and Molecular Biology ›› 1986, Vol. 2 ›› Issue (05) : 83-90.

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ANALYSIS OF THE NUCLEOTlDE SEQUENCE OF CORE ANTIGEN GENE OF Adr SUBTYPE HEPATITIS B VIRUS

Qi,Zu-he Xiong,Wei-jun Yan,Jun Song,Song Yuan,Jian-gang Gai,Liang-wan

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Using the shutgun strategy and cloning of DNA restriction fragments technology,the sequence of HBcAg gene,adr subtype,was determined by dideoxy method and labeled with a35S dATP as radioactive marker.The results indicated that the adr NC-1 core antigen gene is composed of 552 mucleotides from codon ATG to TAG and encoding the polypeptide of 183 amino acids with a molecular weight of 21000 dlt.In comparison with the data published in Japan and Shanghai,there are 1.3-1.8% nucleotides change between adrNC-1 and other adr subtypes corresponding to 0.5% missense mutation,and 9.8-10.9% nucleotides change between adrNC-1 and adw ayw with 3.3-4.4% missense mutation.

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adr Subtype / HBV / HBcAg / HBeAg / Chain termination method / Sequence

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Qi,Zu-he Xiong,Wei-jun Yan,Jun Song,Song Yuan,Jian-gang Gai,Liang-wan. ANALYSIS OF THE NUCLEOTlDE SEQUENCE OF CORE ANTIGEN GENE OF Adr SUBTYPE HEPATITIS B VIRUS[J]. Chinese Journal of Biochemistry and Molecular Biology, 1986, 2(05): 83-90

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Received	Revised	Published
1986-10-20	1986-10-20	1986-10-20
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1986-10-20

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