Purification of T_4 RNA Ligase by Immunoaffinity Chromatography

Hu Mei-hao, Wang Chon-qing, Chen Qing-xuan, and Shen TungDepariment of Biology, Peking University

Chinese Journal of Biochemistry and Molecular Biology ›› 1985, Vol. 1 ›› Issue (04) : 23-28.

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Chinese Journal of Biochemistry and Molecular Biology ›› 1985, Vol. 1 ›› Issue (04) : 23-28.
Article

Purification of T_4 RNA Ligase by Immunoaffinity Chromatography

  • Hu Mei-hao, Wang Chon-qing, Chen Qing-xuan, and Shen TungDepariment of Biology, Peking University
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Abstract

A simplified purification method of the T4 RNA ligase using immunoaffinity chromatography is described. The purified T4 RNA ligase produced one major band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 32pCp and CpGpGpA (or tRNA) were incubated with the purified enzyme and subjected to homochrom atography.No degradation products were found, and this purified enzyme could be used in the synthesis of nucleic acids with defined sequence.

Key words

Ligase / Immunoaffinity chroma tography.

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Hu Mei-hao, Wang Chon-qing, Chen Qing-xuan, and Shen TungDepariment of Biology, Peking University. Purification of T_4 RNA Ligase by Immunoaffinity Chromatography[J]. Chinese Journal of Biochemistry and Molecular Biology, 1985, 1(04): 23-28

References

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